Abstract
The amelogenin gene is the locus of choice for gender identification in forensic science. Here we report on the use of fluorescent DNA melting curve analysis to genotype the amelogenin locus by means of a toehold-assisted DNA strand displacement reaction. The shape of the curves, or "polarity" of the melting peaks, allowed for visual discrimination between male and female DNA samples.
Original language | English |
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Pages | e119-e120 |
DOIs | |
Publication status | Published - 28 Oct 2013 |
Event | 25th Congress of the International Society for Forensic Genetics - Duration: 2 Sep 2013 → … |
Conference
Conference | 25th Congress of the International Society for Forensic Genetics |
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Period | 2/09/13 → … |
Keywords
- Amelogenin
- DNA genotyping
- DNA nanotechnology
- Melting curve analysis
- Toehold displacement