Amelogenin locus typing using toehold-assisted fluorescent DNA melting analysis

Dmitriy Khodakov, Anastasia Khodakova, Adrian Linacre, Amanda Ellis

    Research output: Contribution to conferencePaper

    1 Citation (Scopus)

    Abstract

    The amelogenin gene is the locus of choice for gender identification in forensic science. Here we report on the use of fluorescent DNA melting curve analysis to genotype the amelogenin locus by means of a toehold-assisted DNA strand displacement reaction. The shape of the curves, or "polarity" of the melting peaks, allowed for visual discrimination between male and female DNA samples.

    Original languageEnglish
    Pagese119-e120
    DOIs
    Publication statusPublished - 28 Oct 2013
    Event25th Congress of the International Society for Forensic Genetics -
    Duration: 2 Sep 2013 → …

    Conference

    Conference25th Congress of the International Society for Forensic Genetics
    Period2/09/13 → …

    Keywords

    • Amelogenin
    • DNA genotyping
    • DNA nanotechnology
    • Melting curve analysis
    • Toehold displacement

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  • Cite this

    Khodakov, D., Khodakova, A., Linacre, A., & Ellis, A. (2013). Amelogenin locus typing using toehold-assisted fluorescent DNA melting analysis. e119-e120. Paper presented at 25th Congress of the International Society for Forensic Genetics, . https://doi.org/10.1016/j.fsigss.2013.10.061