Amelogenin locus typing using toehold-assisted fluorescent DNA melting analysis

Dmitriy Khodakov; Anastasia Khodakova; Adrian Linacre; Amanda Ellis

doi:10.1016/j.fsigss.2013.10.061

Amelogenin locus typing using toehold-assisted fluorescent DNA melting analysis

Dmitriy Khodakov, Anastasia Khodakova, Adrian Linacre, Amanda Ellis

Research output: Contribution to conference › Paper › peer-review

1 Citation (Scopus)

Abstract

The amelogenin gene is the locus of choice for gender identification in forensic science. Here we report on the use of fluorescent DNA melting curve analysis to genotype the amelogenin locus by means of a toehold-assisted DNA strand displacement reaction. The shape of the curves, or "polarity" of the melting peaks, allowed for visual discrimination between male and female DNA samples.

Original language	English
Pages	e119-e120
DOIs	https://doi.org/10.1016/j.fsigss.2013.10.061
Publication status	Published - 28 Oct 2013
Event	25th World Congress of the International Society for Forensic Genetics - Melbourne, Australia Duration: 2 Sep 2013 → 7 Sep 2013 Conference number: 25

Conference

Conference	25th World Congress of the International Society for Forensic Genetics
Abbreviated title	ISFG 2013
Country/Territory	Australia
City	Melbourne
Period	2/09/13 → 7/09/13

Keywords

Amelogenin
DNA genotyping
DNA nanotechnology
Melting curve analysis
Toehold displacement

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10.1016/j.fsigss.2013.10.061

Cite this

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title = "Amelogenin locus typing using toehold-assisted fluorescent DNA melting analysis",

abstract = "The amelogenin gene is the locus of choice for gender identification in forensic science. Here we report on the use of fluorescent DNA melting curve analysis to genotype the amelogenin locus by means of a toehold-assisted DNA strand displacement reaction. The shape of the curves, or {"}polarity{"} of the melting peaks, allowed for visual discrimination between male and female DNA samples.",

keywords = "Amelogenin, DNA genotyping, DNA nanotechnology, Melting curve analysis, Toehold displacement",

author = "Dmitriy Khodakov and Anastasia Khodakova and Adrian Linacre and Amanda Ellis",

year = "2013",

month = oct,

day = "28",

doi = "10.1016/j.fsigss.2013.10.061",

language = "English",

pages = "e119--e120",

note = "25th World Congress of the International Society for Forensic Genetics, ISFG 2013 ; Conference date: 02-09-2013 Through 07-09-2013",

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T1 - Amelogenin locus typing using toehold-assisted fluorescent DNA melting analysis

AU - Khodakov, Dmitriy

AU - Khodakova, Anastasia

AU - Linacre, Adrian

AU - Ellis, Amanda

N1 - Conference code: 25

PY - 2013/10/28

Y1 - 2013/10/28

N2 - The amelogenin gene is the locus of choice for gender identification in forensic science. Here we report on the use of fluorescent DNA melting curve analysis to genotype the amelogenin locus by means of a toehold-assisted DNA strand displacement reaction. The shape of the curves, or "polarity" of the melting peaks, allowed for visual discrimination between male and female DNA samples.

AB - The amelogenin gene is the locus of choice for gender identification in forensic science. Here we report on the use of fluorescent DNA melting curve analysis to genotype the amelogenin locus by means of a toehold-assisted DNA strand displacement reaction. The shape of the curves, or "polarity" of the melting peaks, allowed for visual discrimination between male and female DNA samples.

KW - Amelogenin

KW - DNA genotyping

KW - DNA nanotechnology

KW - Melting curve analysis

KW - Toehold displacement

UR - http://www.scopus.com/inward/record.url?scp=84889888680&partnerID=8YFLogxK

U2 - 10.1016/j.fsigss.2013.10.061

DO - 10.1016/j.fsigss.2013.10.061

M3 - Paper

SP - e119-e120

T2 - 25th World Congress of the International Society for Forensic Genetics

Y2 - 2 September 2013 through 7 September 2013

ER -

Amelogenin locus typing using toehold-assisted fluorescent DNA melting analysis

Abstract

Conference

Keywords

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