Amelogenin locus typing using toehold-assisted fluorescent DNA melting analysis

Dmitriy Khodakov; Anastasia Khodakova; Adrian Linacre; Amanda Ellis

doi:10.1016/j.fsigss.2013.10.061

Amelogenin locus typing using toehold-assisted fluorescent DNA melting analysis

Dmitriy Khodakov, Anastasia Khodakova, Adrian Linacre, Amanda Ellis

Research output: Contribution to conference › Paper › peer-review

1 Citation (Scopus)

Abstract

The amelogenin gene is the locus of choice for gender identification in forensic science. Here we report on the use of fluorescent DNA melting curve analysis to genotype the amelogenin locus by means of a toehold-assisted DNA strand displacement reaction. The shape of the curves, or "polarity" of the melting peaks, allowed for visual discrimination between male and female DNA samples.

Original language	English
Pages	e119-e120
DOIs	https://doi.org/10.1016/j.fsigss.2013.10.061
Publication status	Published - 28 Oct 2013
Event	25th Congress of the International Society for Forensic Genetics - Duration: 2 Sep 2013 → …

Conference

Conference	25th Congress of the International Society for Forensic Genetics
Period	2/09/13 → …

Keywords

Amelogenin
DNA genotyping
DNA nanotechnology
Melting curve analysis
Toehold displacement

Access to Document

10.1016/j.fsigss.2013.10.061

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abstract = "The amelogenin gene is the locus of choice for gender identification in forensic science. Here we report on the use of fluorescent DNA melting curve analysis to genotype the amelogenin locus by means of a toehold-assisted DNA strand displacement reaction. The shape of the curves, or {"}polarity{"} of the melting peaks, allowed for visual discrimination between male and female DNA samples.",

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author = "Dmitriy Khodakov and Anastasia Khodakova and Adrian Linacre and Amanda Ellis",

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AU - Linacre, Adrian

AU - Ellis, Amanda

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AB - The amelogenin gene is the locus of choice for gender identification in forensic science. Here we report on the use of fluorescent DNA melting curve analysis to genotype the amelogenin locus by means of a toehold-assisted DNA strand displacement reaction. The shape of the curves, or "polarity" of the melting peaks, allowed for visual discrimination between male and female DNA samples.

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KW - DNA genotyping

KW - DNA nanotechnology

KW - Melting curve analysis

KW - Toehold displacement

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