An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs

Georgia E. Guild; Nicholas G. Paltridge; Meike S. Andersson; James C.R. Stangoulis

doi:10.1007/s11104-017-3352-4

An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs

Georgia E. Guild, Nicholas G. Paltridge, Meike S. Andersson, James C.R. Stangoulis

College of Science and Engineering

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

55 Downloads (Pure)

Abstract

Background and aims: Biofortification breeding programs have the need for rapid and accurate screening methods to identify nutrient dense genotypes. This study explores the use of energy dispersive x-ray fluorescence (EDXRF) for the rapid screening of iron (Fe) and zinc (Zn) concentration in three coarse-grain crops; common bean, maize and cowpea. Methods: Bean, maize and cowpea seed was provided from biofortification breeding programs and analysed with Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) to determine reference Fe and Zn concentrations. A subset of samples for each crop was used to calibrate for Zn and Fe and a separate set of samples used to validate the XRF method. Results: Results indicate that when analysing bean, maize and cowpea flour samples, the EDXRF results were not significantly different to the reference ICP-MS analysis, with an average difference of ± 1 mg kg⁻¹ for both Fe and Zn. Conclusion: EDXRF analysis of common beans, cowpea and maize flour enables rapid and accurate analysis when screening for Fe and Zn in bean, maize and cowpea.

Original language	English
Pages (from-to)	457-466
Number of pages	10
Journal	Plant and Soil
Volume	419
Issue number	1-2
DOIs	https://doi.org/10.1007/s11104-017-3352-4
Publication status	Published - 1 Oct 2017

Bibliographical note

This article is distributed under the terms of theCreative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)

Keywords

Biofortification
EDXRF
Micronutrient
Plant
XRF

Access to Document

10.1007/s11104-017-3352-4

Published versionFinal published version, 1.08 MBLicence: CC BY

Cite this

@article{9d3dbc2042344329bafa64355ec156e4,

title = "An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs",

abstract = "Background and aims: Biofortification breeding programs have the need for rapid and accurate screening methods to identify nutrient dense genotypes. This study explores the use of energy dispersive x-ray fluorescence (EDXRF) for the rapid screening of iron (Fe) and zinc (Zn) concentration in three coarse-grain crops; common bean, maize and cowpea. Methods: Bean, maize and cowpea seed was provided from biofortification breeding programs and analysed with Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) to determine reference Fe and Zn concentrations. A subset of samples for each crop was used to calibrate for Zn and Fe and a separate set of samples used to validate the XRF method. Results: Results indicate that when analysing bean, maize and cowpea flour samples, the EDXRF results were not significantly different to the reference ICP-MS analysis, with an average difference of ± 1 mg kg−1 for both Fe and Zn. Conclusion: EDXRF analysis of common beans, cowpea and maize flour enables rapid and accurate analysis when screening for Fe and Zn in bean, maize and cowpea.",

keywords = "Biofortification, EDXRF, Micronutrient, Plant, XRF",

author = "Guild, {Georgia E.} and Paltridge, {Nicholas G.} and Andersson, {Meike S.} and Stangoulis, {James C.R.}",

note = "This article is distributed under the terms of theCreative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)",

year = "2017",

month = oct,

day = "1",

doi = "10.1007/s11104-017-3352-4",

language = "English",

volume = "419",

pages = "457--466",

journal = "Plant and Soil",

issn = "0032-079X",

publisher = "Springer Nature",

number = "1-2",

}

TY - JOUR

T1 - An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs

AU - Guild, Georgia E.

AU - Paltridge, Nicholas G.

AU - Andersson, Meike S.

AU - Stangoulis, James C.R.

N1 - This article is distributed under the terms of theCreative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Background and aims: Biofortification breeding programs have the need for rapid and accurate screening methods to identify nutrient dense genotypes. This study explores the use of energy dispersive x-ray fluorescence (EDXRF) for the rapid screening of iron (Fe) and zinc (Zn) concentration in three coarse-grain crops; common bean, maize and cowpea. Methods: Bean, maize and cowpea seed was provided from biofortification breeding programs and analysed with Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) to determine reference Fe and Zn concentrations. A subset of samples for each crop was used to calibrate for Zn and Fe and a separate set of samples used to validate the XRF method. Results: Results indicate that when analysing bean, maize and cowpea flour samples, the EDXRF results were not significantly different to the reference ICP-MS analysis, with an average difference of ± 1 mg kg−1 for both Fe and Zn. Conclusion: EDXRF analysis of common beans, cowpea and maize flour enables rapid and accurate analysis when screening for Fe and Zn in bean, maize and cowpea.

AB - Background and aims: Biofortification breeding programs have the need for rapid and accurate screening methods to identify nutrient dense genotypes. This study explores the use of energy dispersive x-ray fluorescence (EDXRF) for the rapid screening of iron (Fe) and zinc (Zn) concentration in three coarse-grain crops; common bean, maize and cowpea. Methods: Bean, maize and cowpea seed was provided from biofortification breeding programs and analysed with Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) to determine reference Fe and Zn concentrations. A subset of samples for each crop was used to calibrate for Zn and Fe and a separate set of samples used to validate the XRF method. Results: Results indicate that when analysing bean, maize and cowpea flour samples, the EDXRF results were not significantly different to the reference ICP-MS analysis, with an average difference of ± 1 mg kg−1 for both Fe and Zn. Conclusion: EDXRF analysis of common beans, cowpea and maize flour enables rapid and accurate analysis when screening for Fe and Zn in bean, maize and cowpea.

KW - Biofortification

KW - EDXRF

KW - Micronutrient

KW - Plant

KW - XRF

UR - http://www.scopus.com/inward/record.url?scp=85026515541&partnerID=8YFLogxK

U2 - 10.1007/s11104-017-3352-4

DO - 10.1007/s11104-017-3352-4

M3 - Article

AN - SCOPUS:85026515541

SN - 0032-079X

VL - 419

SP - 457

EP - 466

JO - Plant and Soil

JF - Plant and Soil

IS - 1-2

ER -

An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this