An enhanced chemiluminescence detection system combined with a modified immunoblot technique for the detection of low molecular weight IgM in sera from healthy adults and neonates

H. J. Xu; K. Umapathysivam; J. McNeilage; T. P. Gordon; P. J. Roberts-Thomson

doi:10.1016/0022-1759(92)90233-J

An enhanced chemiluminescence detection system combined with a modified immunoblot technique for the detection of low molecular weight IgM in sera from healthy adults and neonates

H. J. Xu
, K. Umapathysivam
, J. McNeilage
, T. P. Gordon
, P. J. Roberts-Thomson

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

An enhanced chemiluminescence detection system combined with a modified immunoblot technique is described for the detection of low molecular weight IgM (LMW IgM) in human sera and cell culture supernatants at levels down to 2 pg/ml. This detection system is reliable, specific and more sensitive than the previously described chromogenic detection system. Importantly, this method has, for the first time, revealed LMW IgM in the sera from all 34 healthy adults and 26 neonatal cord bloods tested. A significant linear correlation was observed between the LMW IgM and the total circulating IgM in both the healthy adult sera (r = 0.88, p < 0.001) and cord blood sera (r = 0.98, p < 0.001).

Original language	English
Pages (from-to)	241-247
Number of pages	7
Journal	Journal of Immunological Methods
Volume	146
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(92)90233-J
Publication status	Published - 1992
Externally published	Yes

Keywords

Enhanced chemiluminescence detection system
Healthy adult serum
IgM
Immunoblot
low molecular weight
Neonatal cord blood

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10.1016/0022-1759(92)90233-J

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@article{157059511d5e46a99958b4a242a926e7,

title = "An enhanced chemiluminescence detection system combined with a modified immunoblot technique for the detection of low molecular weight IgM in sera from healthy adults and neonates",

abstract = "An enhanced chemiluminescence detection system combined with a modified immunoblot technique is described for the detection of low molecular weight IgM (LMW IgM) in human sera and cell culture supernatants at levels down to 2 pg/ml. This detection system is reliable, specific and more sensitive than the previously described chromogenic detection system. Importantly, this method has, for the first time, revealed LMW IgM in the sera from all 34 healthy adults and 26 neonatal cord bloods tested. A significant linear correlation was observed between the LMW IgM and the total circulating IgM in both the healthy adult sera (r = 0.88, p < 0.001) and cord blood sera (r = 0.98, p < 0.001).",

keywords = "Enhanced chemiluminescence detection system, Healthy adult serum, IgM, Immunoblot, low molecular weight, Neonatal cord blood",

author = "Xu, \{H. J.\} and K. Umapathysivam and J. McNeilage and Gordon, \{T. P.\} and Roberts-Thomson, \{P. J.\}",

year = "1992",

doi = "10.1016/0022-1759(92)90233-J",

language = "English",

volume = "146",

pages = "241--247",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier B.V.",

number = "2",

}

Xu, HJ, Umapathysivam, K, McNeilage, J, Gordon, TP & Roberts-Thomson, PJ 1992, 'An enhanced chemiluminescence detection system combined with a modified immunoblot technique for the detection of low molecular weight IgM in sera from healthy adults and neonates', Journal of Immunological Methods, vol. 146, no. 2, pp. 241-247. https://doi.org/10.1016/0022-1759(92)90233-J

An enhanced chemiluminescence detection system combined with a modified immunoblot technique for the detection of low molecular weight IgM in sera from healthy adults and neonates. / Xu, H. J.; Umapathysivam, K.; McNeilage, J. et al.
In: Journal of Immunological Methods, Vol. 146, No. 2, 1992, p. 241-247.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - An enhanced chemiluminescence detection system combined with a modified immunoblot technique for the detection of low molecular weight IgM in sera from healthy adults and neonates

AU - Xu, H. J.

AU - Umapathysivam, K.

AU - McNeilage, J.

AU - Gordon, T. P.

AU - Roberts-Thomson, P. J.

PY - 1992

Y1 - 1992

N2 - An enhanced chemiluminescence detection system combined with a modified immunoblot technique is described for the detection of low molecular weight IgM (LMW IgM) in human sera and cell culture supernatants at levels down to 2 pg/ml. This detection system is reliable, specific and more sensitive than the previously described chromogenic detection system. Importantly, this method has, for the first time, revealed LMW IgM in the sera from all 34 healthy adults and 26 neonatal cord bloods tested. A significant linear correlation was observed between the LMW IgM and the total circulating IgM in both the healthy adult sera (r = 0.88, p < 0.001) and cord blood sera (r = 0.98, p < 0.001).

AB - An enhanced chemiluminescence detection system combined with a modified immunoblot technique is described for the detection of low molecular weight IgM (LMW IgM) in human sera and cell culture supernatants at levels down to 2 pg/ml. This detection system is reliable, specific and more sensitive than the previously described chromogenic detection system. Importantly, this method has, for the first time, revealed LMW IgM in the sera from all 34 healthy adults and 26 neonatal cord bloods tested. A significant linear correlation was observed between the LMW IgM and the total circulating IgM in both the healthy adult sera (r = 0.88, p < 0.001) and cord blood sera (r = 0.98, p < 0.001).

KW - Enhanced chemiluminescence detection system

KW - Healthy adult serum

KW - IgM

KW - Immunoblot

KW - low molecular weight

KW - Neonatal cord blood

UR - https://www.scopus.com/pages/publications/0026557058

U2 - 10.1016/0022-1759(92)90233-J

DO - 10.1016/0022-1759(92)90233-J

M3 - Article

C2 - 1538145

SN - 0022-1759

VL - 146

SP - 241

EP - 247

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 2

ER -

An enhanced chemiluminescence detection system combined with a modified immunoblot technique for the detection of low molecular weight IgM in sera from healthy adults and neonates

Abstract

Keywords

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