An Rtt109-independent role for Vps75 in transcription-associated nucleosome dynamics

Luke A. Selth, Yahli Lorch, Maria T. Ocampo-Hafalla, Richard Mitter, Michael Shales, Nevan J. Krogan, Roger D. Kornberg, Jesper Q. Svejstrup

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25 Citations (Scopus)

Abstract

The histone chaperone Vps75 forms a complex with, and stimulates the activity of, the histone acetyltransferase Rtt109. However, Vps75 can also be isolated on its own and might therefore possess Rtt109-independent functions. Analysis of epistatic miniarray profiles showed that VPS75 genetically interacts with factors involved in transcription regulation whereas RTT109 clusters with genes linked to DNA replication/repair. Additional genetic and biochemical experiments revealed a close relationship between Vps75 and RNA polymerase II. Furthermore, Vps75 is recruited to activated genes in an Rtt109-independent manner, and its genome-wide association with genes correlates with transcription rate. Expression microarray analysis identified a number of genes whose normal expression depends on VPS75. Interestingly, histone H2B dynamics at some of these genes are consistent with a role for Vps75 in histone H2A/H2B eviction/deposition during transcription. Indeed, reconstitution of nucleosome disassembly using the ATP-dependent chromatin remodeler Rsc and Vps75 revealed that these proteins can cooperate to remove H2A/H2B dimers from nucleosomes. These results indicate a role for Vps75 in nucleosome dynamics during transcription, and importantly, this function appears to be largely independent of Rtt109.

Original languageEnglish
Pages (from-to)4220-4234
Number of pages15
JournalMolecular and Cellular Biology
Volume29
Issue number15
DOIs
Publication statusPublished - 1 Aug 2009
Externally publishedYes

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    Selth, L. A., Lorch, Y., Ocampo-Hafalla, M. T., Mitter, R., Shales, M., Krogan, N. J., Kornberg, R. D., & Svejstrup, J. Q. (2009). An Rtt109-independent role for Vps75 in transcription-associated nucleosome dynamics. Molecular and Cellular Biology, 29(15), 4220-4234. https://doi.org/10.1128/MCB.01882-08