Analysis of conversion tracts associated with recombination events at the am locus of Neurospora crassa

Frederick J. Bowring; D. E. A. Catcheside

doi:10.1007/s002940050364

Analysis of conversion tracts associated with recombination events at the am locus of Neurospora crassa

Frederick J. Bowring, D. E. A. Catcheside

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

In cross B163, heteroallelic am¹ am⁶ and heterozygous for both conventional genetic flanking markers and closer molecular markers, we previously found that the majority on flanker exchanges were remote from events that generated prototrophic recombinants. We report here that natural polymorphisms distinguishing the parents of cross B163 also include sequences within and closely flanking am. Segregation of these markers in B163 prototrophs confirms that the majority of meiotic recombination events at am resulted from gene conversion. Conversion of am⁶, the distal allele, is more frequent than conversion of am¹. Twelve percent of tracts in am⁶ convertants were discontinuous while 30% of continuous tracts converting am⁶ extend less than 741 bp.

Original language	English
Pages (from-to)	43-49
Number of pages	7
Journal	Current Genetics
Volume	34
Issue number	1
DOIs	https://doi.org/10.1007/s002940050364
Publication status	Published - Jul 1998

Bibliographical note

Funding Information:
Acknowledgements We are indebted to J. A. Kinsey for unpublished sequence data upstream of am. Thanks to Jane Yeadon for helpful comments on the manuscript. This work was supported by a grant from the Australian Research Council.

Keywords

am
Crossing over
Gene conversion
Neurospora

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title = "Analysis of conversion tracts associated with recombination events at the am locus of Neurospora crassa",

abstract = "In cross B163, heteroallelic am1 am6 and heterozygous for both conventional genetic flanking markers and closer molecular markers, we previously found that the majority on flanker exchanges were remote from events that generated prototrophic recombinants. We report here that natural polymorphisms distinguishing the parents of cross B163 also include sequences within and closely flanking am. Segregation of these markers in B163 prototrophs confirms that the majority of meiotic recombination events at am resulted from gene conversion. Conversion of am6, the distal allele, is more frequent than conversion of am1. Twelve percent of tracts in am6 convertants were discontinuous while 30% of continuous tracts converting am6 extend less than 741 bp.",

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T1 - Analysis of conversion tracts associated with recombination events at the am locus of Neurospora crassa

AU - Bowring, Frederick J.

AU - Catcheside, D. E. A.

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N2 - In cross B163, heteroallelic am1 am6 and heterozygous for both conventional genetic flanking markers and closer molecular markers, we previously found that the majority on flanker exchanges were remote from events that generated prototrophic recombinants. We report here that natural polymorphisms distinguishing the parents of cross B163 also include sequences within and closely flanking am. Segregation of these markers in B163 prototrophs confirms that the majority of meiotic recombination events at am resulted from gene conversion. Conversion of am6, the distal allele, is more frequent than conversion of am1. Twelve percent of tracts in am6 convertants were discontinuous while 30% of continuous tracts converting am6 extend less than 741 bp.

AB - In cross B163, heteroallelic am1 am6 and heterozygous for both conventional genetic flanking markers and closer molecular markers, we previously found that the majority on flanker exchanges were remote from events that generated prototrophic recombinants. We report here that natural polymorphisms distinguishing the parents of cross B163 also include sequences within and closely flanking am. Segregation of these markers in B163 prototrophs confirms that the majority of meiotic recombination events at am resulted from gene conversion. Conversion of am6, the distal allele, is more frequent than conversion of am1. Twelve percent of tracts in am6 convertants were discontinuous while 30% of continuous tracts converting am6 extend less than 741 bp.

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Analysis of conversion tracts associated with recombination events at the am locus of Neurospora crassa

Abstract

Bibliographical note

Keywords

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