Analysis of d-penicillamine in plasma by fluorescence derivatisation with N-[p-(2-benzoxazolyl)-phenyl] maleimide and high-performance liquid chromatography

J. O. Miners; I. Fearnley; K. J. Smith; D. J. Birkett; P. M. Brooks; M. W. Whitehouse

doi:10.1016/S0378-4347(00)84347-9

Analysis of d-penicillamine in plasma by fluorescence derivatisation with N-[p-(2-benzoxazolyl)-phenyl] maleimide and high-performance liquid chromatography

J. O. Miners, I. Fearnley, K. J. Smith, D. J. Birkett, P. M. Brooks, M. W. Whitehouse

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

A high-performance liquid chromatographic assay for penicillamine in plasma is described. The method is based on the derivatisation of penicillamine in acidified protein-free plasma supernatants with the sulphydryl-specific reagent N-[p-(2-benzoxazolyl)-phenyl] maleimide (BOPM) to give a stable fluorescent product. After separation of the penicillamine-BOPM derivative by reversed-phase high-performance liquid chromatography, fluorescence detection enables the quantitation of plasma penicillamine concentrations in the range 0.25-500 μmol/l. The method is selective and reproducible, and since chromatography time is less than 7 min the method is readily applicable to the analysis of the large number of samples associated with pharmacokinetic studies.

Original language	English
Pages (from-to)	89-96
Number of pages	8
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	275
DOIs	https://doi.org/10.1016/S0378-4347(00)84347-9
Publication status	Published - 1983

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Miners, J. O., Fearnley, I., Smith, K. J., Birkett, D. J., Brooks, P. M., & Whitehouse, M. W. (1983). Analysis of d-penicillamine in plasma by fluorescence derivatisation with N-[p-(2-benzoxazolyl)-phenyl] maleimide and high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 275, 89-96. https://doi.org/10.1016/S0378-4347(00)84347-9

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title = "Analysis of d-penicillamine in plasma by fluorescence derivatisation with N-[p-(2-benzoxazolyl)-phenyl] maleimide and high-performance liquid chromatography",

abstract = "A high-performance liquid chromatographic assay for penicillamine in plasma is described. The method is based on the derivatisation of penicillamine in acidified protein-free plasma supernatants with the sulphydryl-specific reagent N-[p-(2-benzoxazolyl)-phenyl] maleimide (BOPM) to give a stable fluorescent product. After separation of the penicillamine-BOPM derivative by reversed-phase high-performance liquid chromatography, fluorescence detection enables the quantitation of plasma penicillamine concentrations in the range 0.25-500 μmol/l. The method is selective and reproducible, and since chromatography time is less than 7 min the method is readily applicable to the analysis of the large number of samples associated with pharmacokinetic studies.",

author = "Miners, {J. O.} and I. Fearnley and Smith, {K. J.} and Birkett, {D. J.} and Brooks, {P. M.} and Whitehouse, {M. W.}",

year = "1983",

doi = "10.1016/S0378-4347(00)84347-9",

language = "English",

volume = "275",

pages = "89--96",

journal = "Journal of Chromatography B: Biomedical Sciences and Applications",

issn = "0378-4347",

publisher = "Elsevier B.V.",

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Miners, JO, Fearnley, I, Smith, KJ, Birkett, DJ, Brooks, PM & Whitehouse, MW 1983, 'Analysis of d-penicillamine in plasma by fluorescence derivatisation with N-[p-(2-benzoxazolyl)-phenyl] maleimide and high-performance liquid chromatography', Journal of Chromatography B: Biomedical Sciences and Applications, vol. 275, pp. 89-96. https://doi.org/10.1016/S0378-4347(00)84347-9

Analysis of d-penicillamine in plasma by fluorescence derivatisation with N-[p-(2-benzoxazolyl)-phenyl] maleimide and high-performance liquid chromatography. / Miners, J. O.; Fearnley, I.; Smith, K. J. et al.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 275, 1983, p. 89-96.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Analysis of d-penicillamine in plasma by fluorescence derivatisation with N-[p-(2-benzoxazolyl)-phenyl] maleimide and high-performance liquid chromatography

AU - Miners, J. O.

AU - Fearnley, I.

AU - Smith, K. J.

AU - Birkett, D. J.

AU - Brooks, P. M.

AU - Whitehouse, M. W.

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N2 - A high-performance liquid chromatographic assay for penicillamine in plasma is described. The method is based on the derivatisation of penicillamine in acidified protein-free plasma supernatants with the sulphydryl-specific reagent N-[p-(2-benzoxazolyl)-phenyl] maleimide (BOPM) to give a stable fluorescent product. After separation of the penicillamine-BOPM derivative by reversed-phase high-performance liquid chromatography, fluorescence detection enables the quantitation of plasma penicillamine concentrations in the range 0.25-500 μmol/l. The method is selective and reproducible, and since chromatography time is less than 7 min the method is readily applicable to the analysis of the large number of samples associated with pharmacokinetic studies.

AB - A high-performance liquid chromatographic assay for penicillamine in plasma is described. The method is based on the derivatisation of penicillamine in acidified protein-free plasma supernatants with the sulphydryl-specific reagent N-[p-(2-benzoxazolyl)-phenyl] maleimide (BOPM) to give a stable fluorescent product. After separation of the penicillamine-BOPM derivative by reversed-phase high-performance liquid chromatography, fluorescence detection enables the quantitation of plasma penicillamine concentrations in the range 0.25-500 μmol/l. The method is selective and reproducible, and since chromatography time is less than 7 min the method is readily applicable to the analysis of the large number of samples associated with pharmacokinetic studies.

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JF - Journal of Chromatography B: Biomedical Sciences and Applications

ER -

Analysis of d-penicillamine in plasma by fluorescence derivatisation with N-[p-(2-benzoxazolyl)-phenyl] maleimide and high-performance liquid chromatography

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