A high-performance liquid chromatographic assay for penicillamine in plasma is described. The method is based on the derivatisation of penicillamine in acidified protein-free plasma supernatants with the sulphydryl-specific reagent N-[p-(2-benzoxazolyl)-phenyl] maleimide (BOPM) to give a stable fluorescent product. After separation of the penicillamine-BOPM derivative by reversed-phase high-performance liquid chromatography, fluorescence detection enables the quantitation of plasma penicillamine concentrations in the range 0.25-500 μmol/l. The method is selective and reproducible, and since chromatography time is less than 7 min the method is readily applicable to the analysis of the large number of samples associated with pharmacokinetic studies.
|Number of pages||8|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|Publication status||Published - 1983|