Analysis of d-penicillamine in plasma by fluorescence derivatisation with N-[p-(2-benzoxazolyl)-phenyl] maleimide and high-performance liquid chromatography

J. O. Miners, I. Fearnley, K. J. Smith, D. J. Birkett, P. M. Brooks, M. W. Whitehouse

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    30 Citations (Scopus)

    Abstract

    A high-performance liquid chromatographic assay for penicillamine in plasma is described. The method is based on the derivatisation of penicillamine in acidified protein-free plasma supernatants with the sulphydryl-specific reagent N-[p-(2-benzoxazolyl)-phenyl] maleimide (BOPM) to give a stable fluorescent product. After separation of the penicillamine-BOPM derivative by reversed-phase high-performance liquid chromatography, fluorescence detection enables the quantitation of plasma penicillamine concentrations in the range 0.25-500 μmol/l. The method is selective and reproducible, and since chromatography time is less than 7 min the method is readily applicable to the analysis of the large number of samples associated with pharmacokinetic studies.

    Original languageEnglish
    Pages (from-to)89-96
    Number of pages8
    JournalJournal of Chromatography B: Biomedical Sciences and Applications
    Volume275
    DOIs
    Publication statusPublished - 1983

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