Analysis of low-polarity constituents of reniochalina sp. by gas chromatography-mass spectrometry

Xiaoyan Yan, Yan Jin, Xingju Yu, Wei Zhang, Meifang Jin

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The sponge Reniochalina sp. was collected from China Yellow Sea. The specimen (dry weight 100 g) was repeatedly extracted with cyclohexane. The combined crude extract was then evaporated in vacuo, and the dark red residue (5 g) was subjected to a silica gel column for chromatographic separation using gradient mixtures of ethyl acetate and cyclohexane as eluents. The fraction eluted with ethyl acetate-cyclohexane (1: 9, v/v) was repeatedly separated by preparative liquid chromatography (HiQ sil C18_10 column, 250 mm × 21. 2 mm i. d.) to afford sample B and sample C as dark red oil. These two low-polarity samples were analyzed by gas chromatography-mass spectrometry (GC-MS) equipped with an HP-5 column (30 m × 0.25 mm i. d.). The oven temperature was raised from 40 °C to 250 °C at 10 °C/min and kept at 250 °C for 70 min. Using GC-MS coupled with 1H-nuclear magnetic resonance (1H-NMR), 18 compounds including alkanes, alkenes, fatty acids, fatty acid esters, phthalic acid and phthalic esters, were identified. The results provide to be helpful for in taxonomy of Reniochalina.

Original languageEnglish
Pages (from-to)652-654
Number of pages3
JournalChinese Journal of Chromatography (Se Pu)
Volume22
Issue number6
Publication statusPublished - 30 Nov 2004
Externally publishedYes

Keywords

  • H-nuclear magnetic resonance (H-NMR)
  • Gas chromatography-mass spectrometry
  • Low-polarity constituents
  • Marine sponge Reniochalina sp.

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