TY - JOUR
T1 - Analysis of Pathogenic Bacterial and Yeast Biofilms Using the Combination of Synchrotron ATR-FTIR Microspectroscopy and Chemometric Approaches
AU - Cheeseman, Samuel
AU - Shaw, Z. L.
AU - Vongsvivut, Jitraporn
AU - Crawford, Russell J.
AU - Dupont, Madeleine F.
AU - Boyce, Kylie J.
AU - Gangadoo, Sheeana
AU - Bryant, Saffron J.
AU - Bryant, Gary
AU - Cozzolino, Daniel
AU - Chapman, James
AU - Elbourne, Aaron
AU - Truong, Vi Khanh
PY - 2021/7/1
Y1 - 2021/7/1
N2 - Biofilms are assemblages of microbial cells, extracellular polymeric substances (EPS), and other components extracted from the environment in which they develop. Within biofilms, the spatial distribution of these components can vary. Here we present a fundamental characterization study to show differences between biofilms formed by Gram-positive methicillin-resistant Staphylococcus aureus (MRSA), Gram-negative Pseudomonas aeruginosa, and the yeast-type Candida albicans using synchrotron macro attenuated total reflectance-Fourier transform infrared (ATR-FTIR) micro-spectroscopy. We were able to characterise the pathogenic biofilms’ heterogeneous distribution, which is challenging to do using traditional techniques. Multivariate analyses revealed that the polysaccharides area (1200–950 cm−1) accounted for the most significant variance between biofilm sam-ples, and other spectral regions corresponding to amides, lipids, and polysaccharides all contrib-uted to sample variation. In general, this study will advance our understanding of microbial bio-films and serve as a model for future research on how to use synchrotron source ATR-FTIR micro-spectroscopy to analyse their variations and spatial arrangements.
AB - Biofilms are assemblages of microbial cells, extracellular polymeric substances (EPS), and other components extracted from the environment in which they develop. Within biofilms, the spatial distribution of these components can vary. Here we present a fundamental characterization study to show differences between biofilms formed by Gram-positive methicillin-resistant Staphylococcus aureus (MRSA), Gram-negative Pseudomonas aeruginosa, and the yeast-type Candida albicans using synchrotron macro attenuated total reflectance-Fourier transform infrared (ATR-FTIR) micro-spectroscopy. We were able to characterise the pathogenic biofilms’ heterogeneous distribution, which is challenging to do using traditional techniques. Multivariate analyses revealed that the polysaccharides area (1200–950 cm−1) accounted for the most significant variance between biofilm sam-ples, and other spectral regions corresponding to amides, lipids, and polysaccharides all contrib-uted to sample variation. In general, this study will advance our understanding of microbial bio-films and serve as a model for future research on how to use synchrotron source ATR-FTIR micro-spectroscopy to analyse their variations and spatial arrangements.
KW - ATR
KW - Biofilms
KW - Chemometrics
KW - Infrared
KW - Spatial heterogeneity
KW - Synchrotron
UR - http://www.scopus.com/inward/record.url?scp=85109183545&partnerID=8YFLogxK
U2 - 10.3390/molecules26133890
DO - 10.3390/molecules26133890
M3 - Article
C2 - 34202224
AN - SCOPUS:85109183545
SN - 1420-3049
VL - 26
JO - Molecules
JF - Molecules
IS - 13
M1 - 3890
ER -