Analytical Evaluation of GeneXpert CT/NG, the First Genetic Pointof-Care Assay for Simultaneous Detection of Neisseria gonorrhoeae and Chlamydia trachomatis

Sepehr Tabrizi; Magnus Unemo; Daniel Golparian; Jimmy Twin; E Limnios; Monica Lahra; Rebecca Guy; James Ward; John Kaldor; Basil Donovan; David Wilson; Handan Wand; David Regan; Louise Causer; Belinda Hengel; Lisa Natoli; David Anderson; David Whiley; Mark Shephard; Christopher Fairley

doi:10.1128/JCM.00806-13

Analytical Evaluation of GeneXpert CT/NG, the First Genetic Pointof-Care Assay for Simultaneous Detection of Neisseria gonorrhoeae and Chlamydia trachomatis

Sepehr Tabrizi, Magnus Unemo, Daniel Golparian, Jimmy Twin, E Limnios, Monica Lahra, Rebecca Guy, James Ward, John Kaldor, Basil Donovan, David Wilson, Handan Wand, David Regan, Louise Causer, Belinda Hengel, Lisa Natoli, David Anderson, David Whiley, Mark Shephard, Christopher Fairley

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

GeneXpert CT/NG was evaluated with 372 characterized bacterial strains. Sensitivity of 10 genome copies/reaction was obtained for both agents. Four Neisseria mucosa and two Neisseria subflava isolates were positive for one of two gonococcal targets; however, the assay flagged all as negative. The assay was analytically highly sensitive and specific.

Original language	English
Pages (from-to)	1945-1947
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	51
Issue number	6
DOIs	https://doi.org/10.1128/JCM.00806-13
Publication status	Published - Jun 2013

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Tabrizi, S., Unemo, M., Golparian, D., Twin, J., Limnios, E., Lahra, M., Guy, R., Ward, J., Kaldor, J., Donovan, B., Wilson, D., Wand, H., Regan, D., Causer, L., Hengel, B., Natoli, L., Anderson, D., Whiley, D., Shephard, M., & Fairley, C. (2013). Analytical Evaluation of GeneXpert CT/NG, the First Genetic Pointof-Care Assay for Simultaneous Detection of Neisseria gonorrhoeae and Chlamydia trachomatis. Journal of Clinical Microbiology, 51(6), 1945-1947. https://doi.org/10.1128/JCM.00806-13

Tabrizi, Sepehr ; Unemo, Magnus ; Golparian, Daniel ; Twin, Jimmy ; Limnios, E ; Lahra, Monica ; Guy, Rebecca ; Ward, James ; Kaldor, John ; Donovan, Basil ; Wilson, David ; Wand, Handan ; Regan, David ; Causer, Louise ; Hengel, Belinda ; Natoli, Lisa ; Anderson, David ; Whiley, David ; Shephard, Mark ; Fairley, Christopher. / Analytical Evaluation of GeneXpert CT/NG, the First Genetic Pointof-Care Assay for Simultaneous Detection of Neisseria gonorrhoeae and Chlamydia trachomatis. In: Journal of Clinical Microbiology. 2013 ; Vol. 51, No. 6. pp. 1945-1947.

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title = "Analytical Evaluation of GeneXpert CT/NG, the First Genetic Pointof-Care Assay for Simultaneous Detection of Neisseria gonorrhoeae and Chlamydia trachomatis",

abstract = "GeneXpert CT/NG was evaluated with 372 characterized bacterial strains. Sensitivity of 10 genome copies/reaction was obtained for both agents. Four Neisseria mucosa and two Neisseria subflava isolates were positive for one of two gonococcal targets; however, the assay flagged all as negative. The assay was analytically highly sensitive and specific.",

author = "Sepehr Tabrizi and Magnus Unemo and Daniel Golparian and Jimmy Twin and E Limnios and Monica Lahra and Rebecca Guy and James Ward and John Kaldor and Basil Donovan and David Wilson and Handan Wand and David Regan and Louise Causer and Belinda Hengel and Lisa Natoli and David Anderson and David Whiley and Mark Shephard and Christopher Fairley",

year = "2013",

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doi = "10.1128/JCM.00806-13",

language = "English",

volume = "51",

pages = "1945--1947",

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Tabrizi, S, Unemo, M, Golparian, D, Twin, J, Limnios, E, Lahra, M, Guy, R, Ward, J, Kaldor, J, Donovan, B, Wilson, D, Wand, H, Regan, D, Causer, L, Hengel, B, Natoli, L, Anderson, D, Whiley, D, Shephard, M & Fairley, C 2013, 'Analytical Evaluation of GeneXpert CT/NG, the First Genetic Pointof-Care Assay for Simultaneous Detection of Neisseria gonorrhoeae and Chlamydia trachomatis', Journal of Clinical Microbiology, vol. 51, no. 6, pp. 1945-1947. https://doi.org/10.1128/JCM.00806-13

Analytical Evaluation of GeneXpert CT/NG, the First Genetic Pointof-Care Assay for Simultaneous Detection of Neisseria gonorrhoeae and Chlamydia trachomatis. / Tabrizi, Sepehr; Unemo, Magnus; Golparian, Daniel; Twin, Jimmy; Limnios, E; Lahra, Monica; Guy, Rebecca; Ward, James; Kaldor, John; Donovan, Basil; Wilson, David; Wand, Handan; Regan, David; Causer, Louise; Hengel, Belinda; Natoli, Lisa; Anderson, David; Whiley, David; Shephard, Mark; Fairley, Christopher.

In: Journal of Clinical Microbiology, Vol. 51, No. 6, 06.2013, p. 1945-1947.

Research output: Contribution to journal › Article › peer-review

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AU - Wand, Handan

AU - Regan, David

AU - Causer, Louise

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AB - GeneXpert CT/NG was evaluated with 372 characterized bacterial strains. Sensitivity of 10 genome copies/reaction was obtained for both agents. Four Neisseria mucosa and two Neisseria subflava isolates were positive for one of two gonococcal targets; however, the assay flagged all as negative. The assay was analytically highly sensitive and specific.

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