Antibacterial activity of stevioside towards food-borne pathogenic bacteria

Munish Puri, Deepak Sharma

    Research output: Contribution to journalArticlepeer-review

    30 Citations (Scopus)


    This study determines the inhibitory effect of Stevia rebaudiana leaf extracts and its purified bioactive compound 'stevioside' against food-related pathogens. The S. rebaudiana solvent extracts (1000μg/mL) displayed antibacterial activity to Serratia marcescens, Klebsiella pneumoniae, Bacillus cereus, Pseudomonas aeruginosa, B. subtilis, Alcaligenes denitrificans and Salmonella typhimurium. Of the six solvents, ethanol and acetone extracts displayed the highest zone of inhibition. The bioactive compound from S. rebaudiana was purified by solvent extraction, thin-layer chromatography followed by structural characterization by spectroscopy evidence. Purified stevioside prevented the growth of tested bacterial species, i.e. B. subtilis, K. pneumoniae and S. typhimurium. Significant zone of inhibition (12mm) was observed against B. cereus which proposes potential application of stevioside in foods to increase their shelf life.

    Original languageEnglish
    Pages (from-to)326-329
    Number of pages4
    JournalEngineering in Life Sciences
    Issue number3
    Publication statusPublished - 1 Jun 2011


    • Food borne
    • NMR spectroscopy
    • Pathogen
    • Solvent extraction


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