Antibacterial activity of stevioside towards food-borne pathogenic bacteria

Munish Puri; Deepak Sharma

doi:10.1002/elsc.201000142

Antibacterial activity of stevioside towards food-borne pathogenic bacteria

Munish Puri, Deepak Sharma

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

This study determines the inhibitory effect of Stevia rebaudiana leaf extracts and its purified bioactive compound 'stevioside' against food-related pathogens. The S. rebaudiana solvent extracts (1000μg/mL) displayed antibacterial activity to Serratia marcescens, Klebsiella pneumoniae, Bacillus cereus, Pseudomonas aeruginosa, B. subtilis, Alcaligenes denitrificans and Salmonella typhimurium. Of the six solvents, ethanol and acetone extracts displayed the highest zone of inhibition. The bioactive compound from S. rebaudiana was purified by solvent extraction, thin-layer chromatography followed by structural characterization by spectroscopy evidence. Purified stevioside prevented the growth of tested bacterial species, i.e. B. subtilis, K. pneumoniae and S. typhimurium. Significant zone of inhibition (12mm) was observed against B. cereus which proposes potential application of stevioside in foods to increase their shelf life.

Original language	English
Pages (from-to)	326-329
Number of pages	4
Journal	Engineering in Life Sciences
Volume	11
Issue number	3
DOIs	https://doi.org/10.1002/elsc.201000142
Publication status	Published - 1 Jun 2011

Keywords

Food borne
NMR spectroscopy
Pathogen
Solvent extraction

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AB - This study determines the inhibitory effect of Stevia rebaudiana leaf extracts and its purified bioactive compound 'stevioside' against food-related pathogens. The S. rebaudiana solvent extracts (1000μg/mL) displayed antibacterial activity to Serratia marcescens, Klebsiella pneumoniae, Bacillus cereus, Pseudomonas aeruginosa, B. subtilis, Alcaligenes denitrificans and Salmonella typhimurium. Of the six solvents, ethanol and acetone extracts displayed the highest zone of inhibition. The bioactive compound from S. rebaudiana was purified by solvent extraction, thin-layer chromatography followed by structural characterization by spectroscopy evidence. Purified stevioside prevented the growth of tested bacterial species, i.e. B. subtilis, K. pneumoniae and S. typhimurium. Significant zone of inhibition (12mm) was observed against B. cereus which proposes potential application of stevioside in foods to increase their shelf life.

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Antibacterial activity of stevioside towards food-borne pathogenic bacteria

Abstract

Keywords

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