Antibacterial Nanostructured Surfaces Modulate Protein Adsorption, Inflammatory Responses, and Fibrous Capsule Formation

Rahul Madathiparambil Visalakshan, Richard Bright, Anouck L.S. Burzava, Alex J. Barker, Johanna Simon, Neethu Ninan, Dennis Palms, Jonathan Wood, María Martínez-Negro, Svenja Morsbach, Volker Mailänder, Paul H. Anderson, Toby Brown, Dan Barker, Katharina Landfester, Krasimir Vasilev

Research output: Contribution to journalArticlepeer-review

Abstract

The present study interrogates the interaction of highly efficient antibacterial surfaces containing sharp nanostructures with blood proteins and the subsequent immunological consequences, processes that are of key importance for the fate of every implantable biomaterial. Studies with human serum and plasma pointed to significant differences in the composition of the protein corona that formed on control and nanostructured surfaces. Quantitative analysis using liquid chromatography-mass spectrometry demonstrated that the nanostructured surface attracted more vitronectin and less complement proteins compared to the untreated control. In turn, the protein corona composition modulated the adhesion and cytokine expression by immune cells. Monocytes produced lower amounts of pro-inflammatory cytokines and expressed more anti-inflammatory factors on the nanostructured surface. Studies using an in vivo subcutaneous mouse model showed reduced fibrous capsule thickness which could be a consequence of the attenuated inflammatory response. The results from this work suggest that antibacterial surface modification with sharp spike-like nanostructures may not only lead to the reduction of inflammation but also more favorable foreign body response and enhanced healing, processes that are beneficial for most medical devices implanted in patients.

Original languageEnglish
Pages (from-to)220–235
Number of pages16
JournalACS Applied Materials and Interfaces
Volume15
Issue number1
Early online date23 Nov 2022
DOIs
Publication statusPublished - 11 Jan 2023

Keywords

  • antibacterial surfaces
  • cell attachment
  • fibrous capsule
  • immune response
  • protein adsorption
  • titanium nanostructured surfaces

Fingerprint

Dive into the research topics of 'Antibacterial Nanostructured Surfaces Modulate Protein Adsorption, Inflammatory Responses, and Fibrous Capsule Formation'. Together they form a unique fingerprint.

Cite this