Antibody-Functionalized Porous Silicon Nanoparticles for Vectorization of Hydrophobic Drugs

Emilie Secret, Kevin Smith, Valentina Dubljevic, Eli Moore, Peter MacArdle, Bahman Delalat, Mary-Louise Rogers, Terrance Johns, Jean-Olivier Durand, Frédérique Cunin, Nicolas Voelcker

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    82 Citations (Scopus)

    Abstract

    We describe the preparation of biodegradable porous silicon nanoparticles (pSiNP) functionalized with cancer cell targeting antibodies and loaded with the hydrophobic anti-cancer drug camptothecin. Orientated immobilization of the antibody on the pSiNP is achieved using novel semicarbazide based bioconjugate chemistry. To demonstrate the generality of this targeting approach, the three antibodies MLR2, mAb528 and Rituximab are used, which target neuroblastoma, glioblastoma and B lymphoma cells, respectively. Successful targeting is demonstrated by means of flow cytometry and immunocytochemistry both with cell lines and primary cells. Cell viability assays after incubation with pSiNPs show selective killing of cells expressing the receptor corresponding to the antibody attached on the pSiNP. Mesoporous silicon nanoparticles that are able to specifically target and deliver hydrophobic anti-cancer drugs to cancer cells are developed. Porous silicon nanoparticles are functionalized with antibodies in a controlled way, in order to efficiently target cancer cells expressing the corresponding receptor. High targeting and killing efficiency is demonstrated in vitro on three types on cancer cells: neuroblastoma, glioblastoma and lymphoma cells.

    Original languageEnglish
    Pages (from-to)718-727
    Number of pages10
    JournalAdvanced Healthcare Materials
    Volume2
    Issue number5
    DOIs
    Publication statusPublished - May 2013

    Keywords

    • Antibodies
    • Cancer therapy
    • Drug delivery
    • Nanoparticles
    • Porous silicon
    • Vectorization

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  • Cite this

    Secret, E., Smith, K., Dubljevic, V., Moore, E., MacArdle, P., Delalat, B., Rogers, M-L., Johns, T., Durand, J-O., Cunin, F., & Voelcker, N. (2013). Antibody-Functionalized Porous Silicon Nanoparticles for Vectorization of Hydrophobic Drugs. Advanced Healthcare Materials, 2(5), 718-727. https://doi.org/10.1002/adhm.201200335