Antimicrobial Peptides Grafted onto a Plasma Polymer Interlayer Platform: Performance upon Extended Bacterial Challenge

Stefani S. Griesser, Marek Jasieniak, Krasimir Vasilev, Hans J. Griesser

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)
4 Downloads (Pure)


To combat infections on biomedical devices, antimicrobial coatings have attracted considerable attention, including coatings comprising naturally occurring antimicrobial peptides (AMPs). In this study the aim was to explore performance upon extended challenge by bacteria growing in media above samples. The AMPs LL37, Magainin 2, and Parasin 1 were selected on the basis of well-known membrane disruption activity in solution and were covalently grafted onto a plasma polymer platform, which enables application of this multilayer coating strategy to a wide range of biomaterials. Detailed surface analyses were performed to verify the intended outcomes of the coating sequence. Samples were challenged by incubation in bacterial growth media for 5 and 20 h. Compared with the control plasma polymer surface, all three grafted AMP coatings showed considerable reductions in bacterial colonization even at the high bacterial challenge of initial seeding at 1x107 CFU, but there were increasing numbers of dead bacteria attached to the surface. All three grafted AMP coatings were found to be non-toxic to primary fibroblasts. These coatings thus could be useful to produce antibacterial surface coatings for biomaterials, though possible consequences arising from the presence of dead bacteria need to be studied further, and compared to non-fouling coatings that avoid attachment of dead bacteria.

Original languageEnglish
Article number68
Pages (from-to)1-15
Number of pages15
Issue number1
Publication statusPublished - 2021
Externally publishedYes


  • Antibacterial coating
  • Antimicrobial peptide
  • Bacterial attachment
  • Ll 37
  • Magainin
  • Parasin
  • Plasma polymer


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