Application of direct PCR in forensic casework

Renée Ottens; Jennifer Templeton; Viviana Paradiso; Duncan Taylor; Damien Abarno; Adrian Linacre

doi:10.1016/j.fsigss.2013.10.024

Application of direct PCR in forensic casework

Renée Ottens, Jennifer Templeton, Viviana Paradiso, Duncan Taylor, Damien Abarno, Adrian Linacre

Research output: Contribution to conference › Paper › peer-review

38 Citations (Scopus)

Abstract

Direct PCR is fast becoming a popular method in forensic science due to the advantages of saving time and money in the lab while increasing the probability of obtaining substantial results has a positive rippling effect. A laboratory is able to reduce the time spent on processing trace DNA samples, which can lead to investigators receiving important information in a timely manner that may not have been possible using standard methods. This study highlights the benefits of direct PCR in forensic casework by analysing trace and touch DNA on a range of substrates and exploring the loss of initial DNA due to extraction.

Original language	English
Pages	e47-e48
DOIs	https://doi.org/10.1016/j.fsigss.2013.10.024
Publication status	Published - 7 Nov 2013
Event	25th World Congress of the International Society for Forensic Genetics - Melbourne, Australia Duration: 2 Sept 2013 → 7 Sept 2013 Conference number: 25

Conference

Conference	25th World Congress of the International Society for Forensic Genetics
Abbreviated title	ISFG 2013
Country/Territory	Australia
City	Melbourne
Period	2/09/13 → 7/09/13

Keywords

Direct PCR
Human identification
Latent DNA
Profiling
Short tandem repeat

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10.1016/j.fsigss.2013.10.024

Cite this

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T1 - Application of direct PCR in forensic casework

AU - Ottens, Renée

AU - Templeton, Jennifer

AU - Paradiso, Viviana

AU - Taylor, Duncan

AU - Abarno, Damien

AU - Linacre, Adrian

N1 - Conference code: 25

PY - 2013/11/7

Y1 - 2013/11/7

N2 - Direct PCR is fast becoming a popular method in forensic science due to the advantages of saving time and money in the lab while increasing the probability of obtaining substantial results has a positive rippling effect. A laboratory is able to reduce the time spent on processing trace DNA samples, which can lead to investigators receiving important information in a timely manner that may not have been possible using standard methods. This study highlights the benefits of direct PCR in forensic casework by analysing trace and touch DNA on a range of substrates and exploring the loss of initial DNA due to extraction.

AB - Direct PCR is fast becoming a popular method in forensic science due to the advantages of saving time and money in the lab while increasing the probability of obtaining substantial results has a positive rippling effect. A laboratory is able to reduce the time spent on processing trace DNA samples, which can lead to investigators receiving important information in a timely manner that may not have been possible using standard methods. This study highlights the benefits of direct PCR in forensic casework by analysing trace and touch DNA on a range of substrates and exploring the loss of initial DNA due to extraction.

KW - Direct PCR

KW - Human identification

KW - Latent DNA

KW - Profiling

KW - Short tandem repeat

UR - http://www.scopus.com/inward/record.url?scp=84889884860&partnerID=8YFLogxK

U2 - 10.1016/j.fsigss.2013.10.024

DO - 10.1016/j.fsigss.2013.10.024

M3 - Paper

SP - e47-e48

T2 - 25th World Congress of the International Society for Forensic Genetics

Y2 - 2 September 2013 through 7 September 2013

ER -

Application of direct PCR in forensic casework

Abstract

Conference

Keywords

Access to Document

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