Application of fluorescence resonance energy transfer in protein studies

Linlin Ma, Fan Yang, Jie Zheng

    Research output: Contribution to journalArticlepeer-review

    58 Citations (Scopus)

    Abstract

    Since the physical process of fluorescence resonance energy transfer (FRET) was elucidated more than six decades ago, this peculiar fluorescence phenomenon has turned into a powerful tool for biomedical research due to its compatibility in scale with biological molecules as well as rapid developments in novel fluorophores and optical detection techniques. A wide variety of FRET approaches have been devised, each with its own advantages and drawbacks. Especially in the last decade or so, we are witnessing a flourish of FRET applications in biological investigations, many of which exemplify clever experimental design and rigorous analysis. Here we review the current stage of FRET methods development with the main focus on its applications in protein studies in biological systems, by summarizing the basic components of FRET techniques, most established quantification methods, as well as potential pitfalls, illustrated by example applications.

    Original languageEnglish
    Pages (from-to)87-100
    Number of pages14
    JournalJournal of Molecular Structure
    Volume1077
    DOIs
    Publication statusPublished - 5 Dec 2014

    Keywords

    • Assembly
    • Fluorophore
    • FRET
    • Microscopy
    • Protein
    • Spectroscopy

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