TY - JOUR
T1 - Application of gliadin polymorphism for pedigree analysis in common wheat (Triticum aestivum L.) from Northern Kazakhstan
AU - Utebayev, Maral
AU - Dashkevich, Svetlana
AU - Babkenov, Adylhan
AU - Shtefan, Galina
AU - Fahrudenova, Idiya
AU - Bayahmetova, Sabira
AU - Sharipova, Botagoz
AU - Kaskarbayev, Zheksenbay
AU - Shavrukov, Yuri
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Gliadins, seed storage proteins, are popular markers effectively employed for the analysis of common wheat. Gliadin electrophoretic patterns are genotype-specific, reproducible, not dependent on growing conditions and are suitable for germplasm identification complementary to molecular markers. Gliadins have been identified and used in wheat from various countries, but prior to this study little was known about gliadin polymorphism in wheat from Kazakhstan. In this study, 48 alleles of six gliadin-coding loci were identified in 43 cultivars of spring wheat from Northern Kazakhstan. The alleles Gli-A1f, Gli-B1e, Gli-D1a, Gli-A2p, Gli-B2d and Gli-D2e had maximal frequencies in each of the six loci. Identified Gli alleles in the loci formed ‘Gliadin Genetic Formula’ unique for each studied variety, and these were compared to the published data from previously analyzed wheat varieties. Pedigree analysis of 43 varieties studied for gliadin polymorphisms indicated that some Gli alleles were conserved and inherited from the progenitor cultivar Akmolinka 1. In contrast, other Gli alleles were replaced by those from modern germplasms. It is assumed that a higher frequency of gliadin alleles can be associated with the selection of genotypes with improved traits for yield and seed quality in the studied wheat cultivars from Northern Kazakhstan.
AB - Gliadins, seed storage proteins, are popular markers effectively employed for the analysis of common wheat. Gliadin electrophoretic patterns are genotype-specific, reproducible, not dependent on growing conditions and are suitable for germplasm identification complementary to molecular markers. Gliadins have been identified and used in wheat from various countries, but prior to this study little was known about gliadin polymorphism in wheat from Kazakhstan. In this study, 48 alleles of six gliadin-coding loci were identified in 43 cultivars of spring wheat from Northern Kazakhstan. The alleles Gli-A1f, Gli-B1e, Gli-D1a, Gli-A2p, Gli-B2d and Gli-D2e had maximal frequencies in each of the six loci. Identified Gli alleles in the loci formed ‘Gliadin Genetic Formula’ unique for each studied variety, and these were compared to the published data from previously analyzed wheat varieties. Pedigree analysis of 43 varieties studied for gliadin polymorphisms indicated that some Gli alleles were conserved and inherited from the progenitor cultivar Akmolinka 1. In contrast, other Gli alleles were replaced by those from modern germplasms. It is assumed that a higher frequency of gliadin alleles can be associated with the selection of genotypes with improved traits for yield and seed quality in the studied wheat cultivars from Northern Kazakhstan.
KW - Breeding
KW - Genotype
KW - Gli locus
KW - Gliadin
KW - Gliadin genetic formula
KW - Polymorphism
KW - Wheat
UR - http://www.scopus.com/inward/record.url?scp=84979752698&partnerID=8YFLogxK
U2 - 10.1007/s11738-016-2209-4
DO - 10.1007/s11738-016-2209-4
M3 - Article
SN - 0137-5881
VL - 38
JO - Acta Physiologiae Plantarum
JF - Acta Physiologiae Plantarum
IS - 8
M1 - 204
ER -