Abstract
We report on an optical aptamer sensor for cocaine detection. The cocaine sensitive fluorescein isothiocyanate (FITC)-labeled aptamer underwent a conformational change from a partial single-stranded DNA with a short hairpin to a double-stranded T-junction in the presence of the target. The DNA minor groove binder Hoechst 33342 selectively bound to the double-stranded T-junction, bringing the dye within the Förster radius of FITC, and therefore initiating minor groove binder based energy transfer (MBET), and reporting on the presence of cocaine. The sensor showed a detection limit of 0.2 μM. The sensor was also implemented on a carboxy-functionalized polydimethylsiloxane (PDMS) surface by covalently immobilizing DNA aptamers. The ability of surface-bound cocaine detection is crucial for the development of microfluidic sensors.
Original language | English |
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Pages (from-to) | 76-81 |
Number of pages | 6 |
Journal | Analytica Chimica Acta |
Volume | 719 |
DOIs | |
Publication status | Published - 16 Mar 2012 |
Keywords
- Aptamer
- Cocaine
- Minor groove binder based energy transfer
- Polydimethylsiloxane