Assessing pilot vial material as a surrogate for functional and phenotypic stem cell markers in cryopreserved haematopoietic stem cell product: Bone Marrow Transplant

L. Bai, L. Peters, W. Xia, G. Best, K. Wong, C. Ward, M. Greenwood

Research output: Contribution to journalLetterpeer-review

4 Citations (Scopus)

Abstract

Cryopreservation is often used as a means of storing haematopoietic progenitor cell-apheresis (HPC-A) product when it is collected as part of high-dose therapy for the treatment of haematological malignancies. Processing and cryopreservation of HPC-A is known to reduce the viability and the absolute number of CD34+ cells available for reinfusion.1,2 Therefore, a pilot vial (PV) containing a small aliquot of the same HPC-A material is usually cryopreserved and stored under the same conditions enabling enumeration and viability assessment on post-thaw HPC-A material without disturbing the primary infusion bag required for the transplantation. However, whether PV material can be used to assess other functional or phenotypic stem cell markers in HPC-A material following cryopreservation has not been fully investigated. We aimed to assess whether markers of viability, early apoptosis, DNA damage signalling and oxidative damage, together with CD34+ subset analysis performed similarly between PV and HPC-A material following cryopreservation
Original languageEnglish
Pages (from-to)1631-1632
Number of pages2
JournalBONE MARROW TRANSPLANTATION
Volume51
Issue number12
DOIs
Publication statusPublished - 1 Dec 2016
Externally publishedYes

Keywords

  • Apoptosis Cell Survival Cryopreservation
  • methods DNA Damage Hematopoietic Stem Cells
  • cytology Hodgkin Disease
  • blood/therapy Humans Linear Models Lymphoma, Non-Hodgkin
  • blood
  • therapy Membrane Potentials Multiple Myeloma
  • therapy Oxygen
  • chemistry Phenotype Pilot Projects Reactive Oxygen Species
  • metabolism Signal Transduction Stem Cells
  • cytology

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