Bacterial attachment on optical fibre surfaces

N. Mitik-Dineva, J. Wang, V. K. Truong, P. R. Stoddart, M. R. Alexander, D. J. Albutt, C. Fluke, R. J. Crawford, E. P. Ivanova

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Optical fibres have received considerable attention as high-density sensor arrays suitable for both in vitro and in vivo measurements of biomolecules and biological processes in living organisms and/or nano-environments. The fibre surface was chemically modified by exposure to a selective etchant that preferentially erodes the fibre cores relative to the surrounding cladding material, thus producing a regular pattern of cylindrical wells of approximately 2.5 mum in diameter and 2.5 mum deep. The surface hydrophobicity of the etched and non-etched optical fibres was analysed using the sessile pico-drop method. The surface topography was characterised by atomic force microscopy (AFM), while the surface chemistry was probed by time-of-flight secondary ion mass spectrometry (ToF-SIMS). Six taxonomically different bacterial strains showed a consistent preference for attachment to the nano-scale smoother (R(q) = 273 nm), non-etched fibre surfaces (water contact angle, theta = 106 degrees +/- 4 degrees). In comparison, the surfaces of the etched optical fibres (water contact angle, theta = 96 degrees +/- 10 degrees) were not found to be amenable to bacterial attachment. Bacterial attachment on the non-etched optical fibre substrata varied among different strains.

Original languageEnglish
Pages (from-to)461-470
Number of pages10
Journalbiofouling
Volume26
Issue number4
DOIs
Publication statusPublished - May 2010
Externally publishedYes

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