TY - JOUR
T1 - Balsamin, a novel ribosome-inactivating protein from the seeds of Balsam apple Momordica balsamina
AU - Kaur, Inderdeep
AU - Yadav, Santosh
AU - Hariprasad, Gururao
AU - Gupta, Raghbir
AU - Srinivasan, Alagiri
AU - Batra, Janendra
AU - Puri, Munish
PY - 2012/8
Y1 - 2012/8
N2 - Plant seeds, a rich source of proteins, are considered important for their application as functional ingredients in a food system. A novel ribosome-inactivating protein (RIP), balsamin was purified fromthe seeds of Balsamapple, Momordica balsamina. Balsamin was purified by ion exchange chromatography on CM Sepharose and gel filtration on superdex-75. It has a molecular weight of 28 kDa as shown by SDS-PAGE analysis. Balsamin inhibits protein synthesis in a rabbit reticulocyte lysate-based cell free translation assay with an IC50 of 90.6 ng ml-1. It has RNA N-glycosidase activity and releases a 400-base long fragment termed the Endo fragment from 28S rRNA in the same manner as does saporin-6 from Saponaria officinalis. The N-terminal sequence analysis of the first 12 amino acids of balsamin revealed that it shares83%similarity with type I RIP a-MMC from Momordica charantia and 50% similarity with b-MMC (from Momordica charantia), bryodin I (from Bryonia dioica) and luffin a (from Luffa cylindrica). Balsamin was further characterized by mass spectrometry. CD spectroscopic studies indicate that secondary structure of balsamin contains helix (23.5%), b-strand (24.6%), turn (20%) and random coil (31.9%). Thus RIPs activity expressed in vegetables like Momordica sp. advocates its usage in diet.
AB - Plant seeds, a rich source of proteins, are considered important for their application as functional ingredients in a food system. A novel ribosome-inactivating protein (RIP), balsamin was purified fromthe seeds of Balsamapple, Momordica balsamina. Balsamin was purified by ion exchange chromatography on CM Sepharose and gel filtration on superdex-75. It has a molecular weight of 28 kDa as shown by SDS-PAGE analysis. Balsamin inhibits protein synthesis in a rabbit reticulocyte lysate-based cell free translation assay with an IC50 of 90.6 ng ml-1. It has RNA N-glycosidase activity and releases a 400-base long fragment termed the Endo fragment from 28S rRNA in the same manner as does saporin-6 from Saponaria officinalis. The N-terminal sequence analysis of the first 12 amino acids of balsamin revealed that it shares83%similarity with type I RIP a-MMC from Momordica charantia and 50% similarity with b-MMC (from Momordica charantia), bryodin I (from Bryonia dioica) and luffin a (from Luffa cylindrica). Balsamin was further characterized by mass spectrometry. CD spectroscopic studies indicate that secondary structure of balsamin contains helix (23.5%), b-strand (24.6%), turn (20%) and random coil (31.9%). Thus RIPs activity expressed in vegetables like Momordica sp. advocates its usage in diet.
KW - Balsamin
KW - Cucurbitaceae
KW - Momordica balsamina
KW - Ribosome inactivating protein (RIP)
KW - RNA N-glycosidase
UR - http://www.scopus.com/inward/record.url?scp=84864743826&partnerID=8YFLogxK
U2 - 10.1007/s00726-011-1162-1
DO - 10.1007/s00726-011-1162-1
M3 - Article
VL - 43
SP - 973
EP - 981
JO - AMINO ACIDS
JF - AMINO ACIDS
SN - 0939-4451
IS - 2
ER -