Bead milling for lipid recovery from thraustochytrid cells and selective hydrolysis of Schizochytrium DT3 oil using lipase

Avinesh Byreddy, Colin Barrow, Munish Puri

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)

    Abstract

    Marine microalgae present a renewable alternative source for sustainable production of omega-3 fatty acids, as compared to conventional sources such as krill oil and fish oil. In this study, we optimised a method for lipid extraction from marine thraustochytrids using a bead mill and enzymatic concentration of omega-3 fatty acids from the thraustochytrid oil. The optimised lipid extraction conditions were, bead size 0.4-0.6. μm, 4500. rpm, 4. min of processing time at 5. g biomass concentration. The maximum lipid yield (% dry weight basis) achieved at optimum conditions were 40.5% for Schizochytrium sp. S31 (ATCC) and 49.4% for Schizochytrium sp. DT3 (in-house isolate). DT3 oil contained 39.8% docosahexaenoic acid (DHA) as a percentage of lipid, a higher DHA percentage than S31. Partial hydrolysis of DT3 oil using Candida rugosa lipase was performed to enrich omega-3 polyunsaturated fatty acids (PUFAs) in the glyceride portion. Total omega-3 fatty acid content was increased to 88.7%.

    Original languageEnglish
    Pages (from-to)464-469
    Number of pages6
    JournalBioresource Technology
    Volume200
    DOIs
    Publication statusPublished - 1 Jan 2016

    Keywords

    • Bioactives
    • Candida rugosa
    • Cell disruption
    • Enzymes
    • Schizochytrium

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