Benchmarking Organic Micropollutants in Wastewater, Recycled Water and Drinking Water with In Vitro Bioassays

Beate Escher, Mayumi Allinson, Rolf Altenburger, Peter Bain, Patrick Balaguer, Wibke Busch, Jordan Crago, Nancy Denslow, Elke Dopp, Klara Hilscherova, Andrew Humpage, Anu Kumar, Marina Grimaldi, B Jayasinghe, Barboro Jarosova, Ai Jia, Sergei Makarov, Keith Maruya, Alex Medvedev, Alvine MehintoJamie Mendez, Anita Poulsen, Erik Prochazka, Jessica Richard, Andrea Schifferli, Daniel Schlenk, Stefan Scholz, Fujio Shiraishi, Shane Snyder, Guanyong Su, Janet Tang, Bart van der Burg, Sander van der Linden, Inge Werner, Sandy Westerheide, Chris Wong, Min Yang, Bonnie Yeung, Xiaowei Zhang, Frederic Leusch

    Research output: Contribution to journalArticle

    240 Citations (Scopus)

    Abstract

    Thousands of organic micropollutants and their transformation products occur in water. Although often present at low concentrations, individual compounds contribute to mixture effects. Cell-based bioassays that target health-relevant biological endpoints may therefore complement chemical analysis for water quality assessment. The objective of this study was to evaluate cell-based bioassays for their suitability to benchmark water quality and to assess efficacy of water treatment processes. The selected bioassays cover relevant steps in the toxicity pathways including induction of xenobiotic metabolism, specific and reactive modes of toxic action, activation of adaptive stress response pathways and system responses. Twenty laboratories applied 103 unique in vitro bioassays to a common set of 10 water samples collected in Australia, including wastewater treatment plant effluent, two types of recycled water (reverse osmosis and ozonation/activated carbon filtration), stormwater, surface water, and drinking water. Sixty-five bioassays (63%) showed positive results in at least one sample, typically in wastewater treatment plant effluent, and only five (5%) were positive in the control (ultrapure water). Each water type had a characteristic bioanalytical profile with particular groups of toxicity pathways either consistently responsive or not responsive across test systems. The most responsive health-relevant endpoints were related to xenobiotic metabolism (pregnane X and aryl hydrocarbon receptors), hormone-mediated modes of action (mainly related to the estrogen, glucocorticoid, and antiandrogen activities), reactive modes of action (genotoxicity) and adaptive stress response pathway (oxidative stress response). This study has demonstrated that selected cell-based bioassays are suitable to benchmark water quality and it is recommended to use a purpose-tailored panel of bioassays for routine monitoring.

    Original languageEnglish
    Pages (from-to)1940-1956
    Number of pages17
    JournalEnvironmental Science and Technology
    Volume48
    Issue number3
    DOIs
    Publication statusPublished - 4 Feb 2014

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  • Cite this

    Escher, B., Allinson, M., Altenburger, R., Bain, P., Balaguer, P., Busch, W., Crago, J., Denslow, N., Dopp, E., Hilscherova, K., Humpage, A., Kumar, A., Grimaldi, M., Jayasinghe, B., Jarosova, B., Jia, A., Makarov, S., Maruya, K., Medvedev, A., ... Leusch, F. (2014). Benchmarking Organic Micropollutants in Wastewater, Recycled Water and Drinking Water with In Vitro Bioassays. Environmental Science and Technology, 48(3), 1940-1956. https://doi.org/10.1021/es403899t