Biochemical characterization and molecular insights into substrate recognition of pectin methylesterase from Phytophthora infestans

Rajender Kumar, Sanjiv Kumar, Vincent Bulone, Vaibhav Srivastava

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2 Citations (Scopus)
39 Downloads (Pure)

Abstract

Pectin methylesterases (PMEs) are a class of carbohydrate-active enzymes that act on the O6-methyl ester groups of the homogalacturonan component of pectins, resulting in de-esterification of the substrate polymers and formation of pectate and methanol. PMEs occur in higher plants and microorganisms, including fungi, oomycetes, bacteria, and archaea. Microbial PMEs play a crucial role in pathogens’ invasion of plant tissues. Here, we have determined the structural and functional properties of Pi-PME, a PME from the oomycete plant pathogen Phytophthora infestans. This enzyme exhibits maximum activity at alkaline pH (8.5) and is active over a wide temperature range (25–50 °C). In silico determination of the structure of Pi-PME reveals that the protein consists essentially of three parallel β-sheets interconnected by loops that adopt an overall β-helix organization. The loop regions in the vicinity of the active site are extended compared to plant and fungal PMEs, but they are shorter than the corresponding bacterial and insect regions. Molecular dynamic simulations revealed that Pi-PME interacts most strongly with partially de-methylated homogalacturonans, suggesting that it preferentially uses this type of substrates. The results are compared and discussed with other known PMEs from different organisms, highlighting the specific features of Pi-PME.

Original languageEnglish
Pages (from-to)6023-6032
Number of pages10
JournalComputational and Structural Biotechnology Journal
Volume20
Early online date4 Nov 2022
DOIs
Publication statusPublished - 2022

Keywords

  • Molecular simulation
  • Oomycete
  • Pectin methylesterases
  • Phytophthora infestans
  • Potato late blight

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