Bioethanol production potential of a novel thermophilic isolate Thermoanaerobacter sp. DBT-IOC-X2 isolated from Chumathang hot spring

Nisha Singh, Munish Puri, Deepak Tuli, Ravi Gupta, Colin Barrow, Anshu Mathur

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Dilute acid pretreatment of biomass generates enormous amount of hydrolysate (rich in inhibitors and pentose sugars), that remains unutilized for bioethanol production due to inadequacy of efficient C 5 -fermenting organisms. In this study, a predominantly pentose fermenting extremely thermophilic bacterium strain DBT-IOC-X2, pertaining to the genus Thermoanaerobacter was isolated from Himalayan hot spring. Batch experiments indicated substantial inhibitor resistance (2 g dm −3 for furfural, 5-HMF, and acetic acid), substrate tolerance (∼15 g dm −3 ), co-sugar fermentation ability (co-production ethanol yield of 0.29 g/g), and high ethanol yield (83.57% and 91.12% of the theoretical maximum from 5 g dm −3 glucose and xylose, respectively) by the bacterium at 70 °C and pH 8.0. Here, bioethanol production process was developed using pre-treated rice straw hydrolysate (PRSH) as low-cost agro-waste and 83.47% of the total sugar conversion was obtained. This study shows that Thermoanaerobacter sp. DBT-IOC-X2 could utilize diluted PRSH efficiently to improve the overall cost-effectiveness of biomass processing to bioethanol.

Original languageEnglish
Pages (from-to)122-130
Number of pages9
JournalBIOMASS & BIOENERGY
Volume116
Issue number2018
DOIs
Publication statusPublished - Sep 2018

Keywords

  • Bioethanol
  • Hot spring
  • Pentose-rich sugar hydrolysate
  • Rice straw
  • Thermoanaerobacter

Fingerprint Dive into the research topics of 'Bioethanol production potential of a novel thermophilic isolate Thermoanaerobacter sp. DBT-IOC-X2 isolated from Chumathang hot spring'. Together they form a unique fingerprint.

Cite this