Biofortified indica rice attains iron and zinc nutrition dietary targets in the field

Kurniawan Trijatmiko; Conrado Dueñas; Nikolaos Tsakirpaloglou; Lina Torrizo; Felichi Arines; Cheryl Adeva; Jeanette Balindong; Norman Oliva; Maria Sapasap; Jaime Borrero; Jessica Rey; Perigio Francisco; Andy Nelson; Hiromi Nakanishi; Enzo Lombi; Elad Tako; Raymond Glahn; James Stangoulis; Prabhjit Chadha-Mohanty; Alexander Johnson; Joe Tohme; Gerard Barry; Inez Slamet-Loedin

doi:10.1038/srep19792

Biofortified indica rice attains iron and zinc nutrition dietary targets in the field

Kurniawan Trijatmiko, Conrado Dueñas, Nikolaos Tsakirpaloglou, Lina Torrizo, Felichi Arines, Cheryl Adeva, Jeanette Balindong, Norman Oliva, Maria Sapasap, Jaime Borrero, Jessica Rey, Perigio Francisco, Andy Nelson, Hiromi Nakanishi, Enzo Lombi, Elad Tako, Raymond Glahn, James Stangoulis, Prabhjit Chadha-Mohanty, Alexander JohnsonJoe Tohme, Gerard Barry, Inez Slamet-Loedin

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Abstract

More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g^-1 iron (Fe) and 16 μg g^-1 zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g^-1 Fe and 28 μg g^-1 Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g^-1 in glasshouse settings; in contrast, under field conditions, 4 μg g^-1 was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g^-1 Fe and 45.7 μg g^-1 Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds.

Original language	English
Article number	19792
Pages (from-to)	Art: 19792
Number of pages	13
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep19792
Publication status	Published - 25 Jan 2016

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Trijatmiko, K., Dueñas, C., Tsakirpaloglou, N., Torrizo, L., Arines, F., Adeva, C., Balindong, J., Oliva, N., Sapasap, M., Borrero, J., Rey, J., Francisco, P., Nelson, A., Nakanishi, H., Lombi, E., Tako, E., Glahn, R., Stangoulis, J., Chadha-Mohanty, P., ... Slamet-Loedin, I. (2016). Biofortified indica rice attains iron and zinc nutrition dietary targets in the field. Scientific Reports, 6, Art: 19792. Article 19792. https://doi.org/10.1038/srep19792

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title = "Biofortified indica rice attains iron and zinc nutrition dietary targets in the field",

abstract = "More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g-1 iron (Fe) and 16 μg g-1 zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g-1 Fe and 28 μg g-1 Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g-1 in glasshouse settings; in contrast, under field conditions, 4 μg g-1 was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g-1 Fe and 45.7 μg g-1 Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds.",

author = "Kurniawan Trijatmiko and Conrado Due{\~n}as and Nikolaos Tsakirpaloglou and Lina Torrizo and Felichi Arines and Cheryl Adeva and Jeanette Balindong and Norman Oliva and Maria Sapasap and Jaime Borrero and Jessica Rey and Perigio Francisco and Andy Nelson and Hiromi Nakanishi and Enzo Lombi and Elad Tako and Raymond Glahn and James Stangoulis and Prabhjit Chadha-Mohanty and Alexander Johnson and Joe Tohme and Gerard Barry and Inez Slamet-Loedin",

year = "2016",

month = jan,

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doi = "10.1038/srep19792",

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Trijatmiko, K, Dueñas, C, Tsakirpaloglou, N, Torrizo, L, Arines, F, Adeva, C, Balindong, J, Oliva, N, Sapasap, M, Borrero, J, Rey, J, Francisco, P, Nelson, A, Nakanishi, H, Lombi, E, Tako, E, Glahn, R, Stangoulis, J, Chadha-Mohanty, P, Johnson, A, Tohme, J, Barry, G & Slamet-Loedin, I 2016, 'Biofortified indica rice attains iron and zinc nutrition dietary targets in the field', Scientific Reports, vol. 6, 19792, pp. Art: 19792. https://doi.org/10.1038/srep19792

TY - JOUR

T1 - Biofortified indica rice attains iron and zinc nutrition dietary targets in the field

AU - Trijatmiko, Kurniawan

AU - Dueñas, Conrado

AU - Tsakirpaloglou, Nikolaos

AU - Torrizo, Lina

AU - Arines, Felichi

AU - Adeva, Cheryl

AU - Balindong, Jeanette

AU - Oliva, Norman

AU - Sapasap, Maria

AU - Borrero, Jaime

AU - Rey, Jessica

AU - Francisco, Perigio

AU - Nelson, Andy

AU - Nakanishi, Hiromi

AU - Lombi, Enzo

AU - Tako, Elad

AU - Glahn, Raymond

AU - Stangoulis, James

AU - Chadha-Mohanty, Prabhjit

AU - Johnson, Alexander

AU - Tohme, Joe

AU - Barry, Gerard

AU - Slamet-Loedin, Inez

PY - 2016/1/25

Y1 - 2016/1/25

N2 - More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g-1 iron (Fe) and 16 μg g-1 zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g-1 Fe and 28 μg g-1 Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g-1 in glasshouse settings; in contrast, under field conditions, 4 μg g-1 was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g-1 Fe and 45.7 μg g-1 Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds.

AB - More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g-1 iron (Fe) and 16 μg g-1 zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g-1 Fe and 28 μg g-1 Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g-1 in glasshouse settings; in contrast, under field conditions, 4 μg g-1 was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g-1 Fe and 45.7 μg g-1 Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds.

UR - http://www.scopus.com/inward/record.url?scp=84955481397&partnerID=8YFLogxK

U2 - 10.1038/srep19792

DO - 10.1038/srep19792

M3 - Article

SN - 2045-2322

VL - 6

SP - Art: 19792

JO - Scientific Reports

JF - Scientific Reports

M1 - 19792

ER -

Biofortified indica rice attains iron and zinc nutrition dietary targets in the field

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