Biomimetic Bacterium-like Particles Loaded with Aggregation-Induced Emission Photosensitizers as Plasma Coatings for Implant-Associated Infections

Jianzhong Wang, Neethu Ninan, Ngoc Huu Nguyen, Manh Tuong Nguyen, Resmarani Sahu, Tien Thanh Nguyen, Agnieszka Mierczynska-Vasilev, Krasimir Vasilev, Vi Khanh Truong, Youhong Tang

Research output: Contribution to journalArticlepeer-review

Abstract

Developing novel antibacterial strategies has become an urgent requisite to overcome the increasing pervasiveness of antimicrobial-resistant bacteria and the advent of biofilms. Aggregation-induced emission-based photosensitizers (AIE PSs) are promising candidates due to their unique photodynamic and photothermal properties. Bioengineering structure-inherent AIE PSs for developing thin film coatings is still an unexplored area in the field of nanoscience. We have adopted a synergistic approach combining plasma technology and AIE PS-based photodynamic therapy to develop coatings that can eradicate bacterial infections. Here, we loaded AIE PSs within biomimetic bacterium-like particles derived from a probiotic strain, Lactobacillus fermentum. These hybrid conjugates are then immobilized on polyoxazoline-coated substrates to develop a bioinspired coating to fight against implant-associated infections. These coatings could selectively kill Gram-positive and Gram-negative bacteria, but not damage mammalian cells. The mechanistic studies revealed that the coatings can generate reactive oxygen species that can rupture the bacterial cell membranes. The mRNA gene expression of proinflammatory cytokines confirmed that they can modulate infection-related immune responses. Thus, this nature-inspired design has opened a new avenue for the fabrication of a next-generation antibacterial coating to reduce infections and associated burdens.

Original languageEnglish
Pages (from-to)18449-18458
Number of pages10
JournalACS Applied Materials and Interfaces
Volume16
Issue number15
Early online date5 Apr 2024
DOIs
Publication statusPublished - 17 Apr 2024

Keywords

  • aggregation-induced emission
  • antibacterial
  • bacterium-like particles
  • plasma coating
  • reactive oxygen species

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