Bioturbation of peanut worms Sipunculus nudus on the composition of prokaryotic communities in a tidal flat as revealed by 16S rRNA gene sequences

Junwei Li, Ruiping Hu, Yongjian Guo, Suwen Chen, Xiaoyong Xie, Jian G. Qin, Zhenhua Ma, Changbo Zhu, Surui Pei

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Abstract

To understand the impacts of peanut worms Sipunculus nudus on the prokaryotic community composition in a tidal flat, an onsite investigation was conducted in Suixi in the Beibu Gulf (109.82E, 21.35N) in the burrow sediments, non-burrow sediments and the sediments without peanut worm disturbance (control). The16S rRNA gene Illumina MiSeq sequencing was used to investigate the microbial communities and their response to bioturbation by S. nudus in a sandy tidal flat. A total of 18 bacteria phyla were detected, and Proteobacteria and Cyanobacteria constituted the majority of the prokaryotic community in the samples. The distribution of the relative abundances of genera showed that approximately 6.99%–17% of the reads in the samples were classified into 25 known genera. Sulfate-reducing bacteria (Desulfococcus and Desulfosarcina) were the most abundant taxa, followed by Thermodesulfovibrionaceae LCP-6, indicating that sulfate reduction is the main process in the sandy tidal flat. The abundances of Desulfococcus, LCP-6 and Cyanobacterium in the non-burrow sediment were greater than in the burrow sediment, suggesting that the anoxic condition is more suitable for Desulfococcus and LCP-6 when the activity of S. nudus is absent. The biomass of Cyanobacterium was decreased by the feeding bioturbation of S. nudus. Meanwhile, the relative abundance of the Bacteroidetes Luteimonas in the burrow sediments was significantly greater than in the non-burrow sediment, and there was a strong relationship between S. nudus bioturbation and increased in oxygen contents and oxidation-reduction potentials in the burrow sediment. The abundances of Desulfococcus and LCP-6 were greater in the middle layer (20–30 cm) than in the top layer in the non-burrow sediment. However, the middle and bottom layers (20–30, 30–40 cm) had higher abundances of these genera than did the upper layers (0–10, 10–20 cm) in the burrow sediments. The abundances of the Fusobacteria Propionigenium and the Spirochaetes Spirochaeta were greater in the middle and bottom layers (20–30 cm, 30–40 cm) than in the top layers (0–10, 10–20 cm) in the burrow sediment, but this phenomenon was not found in the non-burrow sediment. This study demonstrates that bioturbation by S. nudus plays an important role in reshaping the bacterial community composition in intertidal regions.

Original languageEnglish
Article numbere00802
JournalMicrobiologyOpen
Volume8
Issue number8
DOIs
Publication statusPublished - 7 Feb 2019

Bibliographical note

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium,
provided the original work is properly cited.
© 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

Keywords

  • bacterial community
  • high-throughput 16S rRNA gene sequencing
  • sandy tidal flat
  • Sipunculus nudus Linnaeus

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