Building a plant DNA barcode reference library for a diverse tropical flora: an example from Queensland, Australia

Craig M. Costion, Andrew J. Lowe, Maurizio Rossetto, Robert M. Kooyman, Martin F. Breed, Andrew Ford, Darren M. Crayn

Research output: Contribution to journalArticle

5 Citations (Scopus)
1 Downloads (Pure)

Abstract

A foundation for a DNA barcode reference library for the tropical plants of Australia is presented here. A total of 1572 DNA barcode sequences are compiled from 848 tropical Queensland species. The dataset represents 35% of the total flora of Queensland's Wet Tropics Bioregion, 57% of its tree species and 28% of the shrub species. For approximately half of the sampled species, we investigated the occurrence of infraspecific molecular variation in DNA barcode loci rbcLa, matK, and the trnH-psbA intergenic spacer region across previously recognized biogeographic barriers. We found preliminary support for the notion that DNA barcode reference libraries can be used as a tool for inferring biogeographic patterns at regional scales. It is expected that this dataset will find applications in taxonomic, ecological, and applied conservation research.

Original languageEnglish
Article number5
Number of pages9
JournalDiversity
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Mar 2016
Externally publishedYes

Bibliographical note

© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons by Attribution
(CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

Keywords

  • Biogeography
  • matK
  • rbcLa
  • trnH-psbA intergenic spacer
  • Wet tropics

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