Building a plant DNA barcode reference library for a diverse tropical flora: an example from Queensland, Australia

Craig M. Costion; Andrew J. Lowe; Maurizio Rossetto; Robert M. Kooyman; Martin F. Breed; Andrew Ford; Darren M. Crayn

doi:10.3390/d8010005

Building a plant DNA barcode reference library for a diverse tropical flora: an example from Queensland, Australia

Craig M. Costion, Andrew J. Lowe, Maurizio Rossetto, Robert M. Kooyman, Martin F. Breed, Andrew Ford, Darren M. Crayn

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Abstract

A foundation for a DNA barcode reference library for the tropical plants of Australia is presented here. A total of 1572 DNA barcode sequences are compiled from 848 tropical Queensland species. The dataset represents 35% of the total flora of Queensland's Wet Tropics Bioregion, 57% of its tree species and 28% of the shrub species. For approximately half of the sampled species, we investigated the occurrence of infraspecific molecular variation in DNA barcode loci rbcLa, matK, and the trnH-psbA intergenic spacer region across previously recognized biogeographic barriers. We found preliminary support for the notion that DNA barcode reference libraries can be used as a tool for inferring biogeographic patterns at regional scales. It is expected that this dataset will find applications in taxonomic, ecological, and applied conservation research.

Original language	English
Article number	5
Number of pages	9
Journal	Diversity
Volume	8
Issue number	1
DOIs	https://doi.org/10.3390/d8010005
Publication status	Published - 1 Mar 2016
Externally published	Yes

Bibliographical note

© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons by Attribution
(CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

Keywords

Biogeography
matK
rbcLa
trnH-psbA intergenic spacer
Wet tropics

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10.3390/d8010005Licence: CC BY

Costion_Building_P2016Final published version, 833 KBLicence: CC BY

Cite this

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title = "Building a plant DNA barcode reference library for a diverse tropical flora: an example from Queensland, Australia",

abstract = "A foundation for a DNA barcode reference library for the tropical plants of Australia is presented here. A total of 1572 DNA barcode sequences are compiled from 848 tropical Queensland species. The dataset represents 35% of the total flora of Queensland's Wet Tropics Bioregion, 57% of its tree species and 28% of the shrub species. For approximately half of the sampled species, we investigated the occurrence of infraspecific molecular variation in DNA barcode loci rbcLa, matK, and the trnH-psbA intergenic spacer region across previously recognized biogeographic barriers. We found preliminary support for the notion that DNA barcode reference libraries can be used as a tool for inferring biogeographic patterns at regional scales. It is expected that this dataset will find applications in taxonomic, ecological, and applied conservation research.",

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AU - Breed, Martin F.

AU - Ford, Andrew

AU - Crayn, Darren M.

N1 - © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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AB - A foundation for a DNA barcode reference library for the tropical plants of Australia is presented here. A total of 1572 DNA barcode sequences are compiled from 848 tropical Queensland species. The dataset represents 35% of the total flora of Queensland's Wet Tropics Bioregion, 57% of its tree species and 28% of the shrub species. For approximately half of the sampled species, we investigated the occurrence of infraspecific molecular variation in DNA barcode loci rbcLa, matK, and the trnH-psbA intergenic spacer region across previously recognized biogeographic barriers. We found preliminary support for the notion that DNA barcode reference libraries can be used as a tool for inferring biogeographic patterns at regional scales. It is expected that this dataset will find applications in taxonomic, ecological, and applied conservation research.

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JO - Diversity

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ER -

Building a plant DNA barcode reference library for a diverse tropical flora: an example from Queensland, Australia

Abstract

Bibliographical note

Keywords

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Cite this