BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei

Jolene Bowers, David Engelthaler, Jennifer Ginther, Tamila Pearson, Sharon Peacock, Apichai Tuanyok, David Wagner, Bart Currie, Paul Keim

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    Abstract

    A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.

    Original languageEnglish
    Article numbere15413
    Pages (from-to)15413
    Number of pages5
    JournalPLoS One
    Volume5
    Issue number11
    DOIs
    Publication statusPublished - 2010

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    Bowers, J., Engelthaler, D., Ginther, J., Pearson, T., Peacock, S., Tuanyok, A., Wagner, D., Currie, B., & Keim, P. (2010). BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei. PLoS One, 5(11), 15413. [e15413]. https://doi.org/10.1371/journal.pone.0015413