BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei

Jolene Bowers; David Engelthaler; Jennifer Ginther; Tamila Pearson; Sharon Peacock; Apichai Tuanyok; David Wagner; Bart Currie; Paul Keim

doi:10.1371/journal.pone.0015413

BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei

Jolene Bowers, David Engelthaler, Jennifer Ginther, Tamila Pearson, Sharon Peacock, Apichai Tuanyok, David Wagner, Bart Currie, Paul Keim

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.

Original language	English
Article number	e15413
Pages (from-to)	15413
Number of pages	5
Journal	PLoS One
Volume	5
Issue number	11
DOIs	https://doi.org/10.1371/journal.pone.0015413
Publication status	Published - 2010

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AU - Bowers, Jolene

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AU - Pearson, Tamila

AU - Peacock, Sharon

AU - Tuanyok, Apichai

AU - Wagner, David

AU - Currie, Bart

AU - Keim, Paul

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AB - A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.

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BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei

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