Carbon-Fiber Amperometry in the Study of Exocytosis

Michael Duffield, RaviNarayan Raghupathi, Damien Keating

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    Understanding how signaling molecules are released from cells is essential for furthering our knowledge of the basic biological mechanisms controlling many significant biological pathways. These molecules, including neurotransmitters, hormones, growth factors, and peptides, are released from cells via a process called exocytosis. Our laboratory has utilized a noninvasive method of measuring the release of oxidizable molecules from cells, known as carbon-fiber amperometry. In this chapter we will describe how we undertake such measurements, how the resulting data is analyzed, and what the outcomes mean in terms of physiology. We provide examples of our work measuring catecholamine release in single chromaffin cells as well as serotonin release from intact sections of colon.

    Original languageEnglish
    Title of host publicationExocytosis Methods
    PublisherHumana Press: Springer
    Pages39-54
    Number of pages16
    Volume83
    ISBN (Print)9781627036757
    DOIs
    Publication statusPublished - 2014

    Publication series

    NameNeuromethods
    Volume83
    ISSN (Print)0893-2336
    ISSN (Electronic)1940-6045

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  • Cite this

    Duffield, M., Raghupathi, R., & Keating, D. (2014). Carbon-Fiber Amperometry in the Study of Exocytosis. In Exocytosis Methods (Vol. 83, pp. 39-54). (Neuromethods; Vol. 83). Humana Press: Springer. https://doi.org/10.1007/978-1-62703-676-4_3