Case report: Identification of intra-laboratory blood culture contamination with Staphylococcus aureus by whole genome sequencing

Anushia Ashokan; Lito Electra Papanicolas; Lex Ee Leong; Maria Theodossi; Santhosh Daniel; Steven Lodewijk Wesselingh; Geraint Berian Rogers; David Llewellyn Gordon

doi:10.1016/j.diagmicrobio.2019.02.016

Case report: Identification of intra-laboratory blood culture contamination with Staphylococcus aureus by whole genome sequencing

Anushia Ashokan, Lito Electra Papanicolas, Lex Ee Leong, Maria Theodossi, Santhosh Daniel, Steven Lodewijk Wesselingh, Geraint Berian Rogers, David Llewellyn Gordon

College of Medicine and Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

Staphylococcus aureus in blood cultures is rarely considered a contaminant. We report a case of intra-laboratory contamination between blood culture bottles which was confirmed by whole genome sequencing, highlighting the importance of molecular analysis in the clinical laboratory setting.

Original language	English
Pages (from-to)	331-333
Number of pages	3
Journal	Diagnostic Microbiology and Infectious Disease
Volume	94
Issue number	4
DOIs	https://doi.org/10.1016/j.diagmicrobio.2019.02.016
Publication status	Published - Aug 2019

Keywords

clinical isolates
metagenomics
MRSA
bacteremia
Metagenomics
Clinical isolates
Bacteremia

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Ashokan, A., Papanicolas, L. E., Leong, L. E., Theodossi, M., Daniel, S., Wesselingh, S. L., Rogers, G. B., & Gordon, D. L. (2019). Case report: Identification of intra-laboratory blood culture contamination with Staphylococcus aureus by whole genome sequencing. Diagnostic Microbiology and Infectious Disease, 94(4), 331-333. https://doi.org/10.1016/j.diagmicrobio.2019.02.016

Ashokan, Anushia ; Papanicolas, Lito Electra ; Leong, Lex Ee ; Theodossi, Maria ; Daniel, Santhosh ; Wesselingh, Steven Lodewijk ; Rogers, Geraint Berian ; Gordon, David Llewellyn. / Case report : Identification of intra-laboratory blood culture contamination with Staphylococcus aureus by whole genome sequencing. In: Diagnostic Microbiology and Infectious Disease. 2019 ; Vol. 94, No. 4. pp. 331-333.

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title = "Case report: Identification of intra-laboratory blood culture contamination with Staphylococcus aureus by whole genome sequencing",

abstract = "Staphylococcus aureus in blood cultures is rarely considered a contaminant. We report a case of intra-laboratory contamination between blood culture bottles which was confirmed by whole genome sequencing, highlighting the importance of molecular analysis in the clinical laboratory setting.",

keywords = "clinical isolates, metagenomics, MRSA, bacteremia, Metagenomics, Clinical isolates, Bacteremia",

author = "Anushia Ashokan and Papanicolas, {Lito Electra} and Leong, {Lex Ee} and Maria Theodossi and Santhosh Daniel and Wesselingh, {Steven Lodewijk} and Rogers, {Geraint Berian} and Gordon, {David Llewellyn}",

year = "2019",

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doi = "10.1016/j.diagmicrobio.2019.02.016",

language = "English",

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Ashokan, A, Papanicolas, LE, Leong, LE, Theodossi, M, Daniel, S, Wesselingh, SL , Rogers, GB & Gordon, DL 2019, 'Case report: Identification of intra-laboratory blood culture contamination with Staphylococcus aureus by whole genome sequencing', Diagnostic Microbiology and Infectious Disease, vol. 94, no. 4, pp. 331-333. https://doi.org/10.1016/j.diagmicrobio.2019.02.016

Case report : Identification of intra-laboratory blood culture contamination with Staphylococcus aureus by whole genome sequencing. / Ashokan, Anushia; Papanicolas, Lito Electra; Leong, Lex Ee; Theodossi, Maria; Daniel, Santhosh; Wesselingh, Steven Lodewijk ; Rogers, Geraint Berian ; Gordon, David Llewellyn.

In: Diagnostic Microbiology and Infectious Disease, Vol. 94, No. 4, 08.2019, p. 331-333.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Case report

T2 - Identification of intra-laboratory blood culture contamination with Staphylococcus aureus by whole genome sequencing

AU - Ashokan, Anushia

AU - Papanicolas, Lito Electra

AU - Leong, Lex Ee

AU - Theodossi, Maria

AU - Daniel, Santhosh

AU - Wesselingh, Steven Lodewijk

AU - Rogers, Geraint Berian

AU - Gordon, David Llewellyn

PY - 2019/8

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N2 - Staphylococcus aureus in blood cultures is rarely considered a contaminant. We report a case of intra-laboratory contamination between blood culture bottles which was confirmed by whole genome sequencing, highlighting the importance of molecular analysis in the clinical laboratory setting.

AB - Staphylococcus aureus in blood cultures is rarely considered a contaminant. We report a case of intra-laboratory contamination between blood culture bottles which was confirmed by whole genome sequencing, highlighting the importance of molecular analysis in the clinical laboratory setting.

KW - clinical isolates

KW - metagenomics

KW - MRSA

KW - bacteremia

KW - Metagenomics

KW - Clinical isolates

KW - Bacteremia

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DO - 10.1016/j.diagmicrobio.2019.02.016

M3 - Article

VL - 94

SP - 331

EP - 333

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 4

ER -

Case report: Identification of intra-laboratory blood culture contamination with Staphylococcus aureus by whole genome sequencing

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Keywords

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