CD44 regulates dendrite morphogenesis through Src tyrosine kinase-dependent positioning of the Golgi

Anna Skupien; Anna Konopka; Pawe I. Trzaskoma; Josephine Labus; Adam Gorlewicz; Lukasz Swiech; Matylda Babraj; Hubert Dolezyczek; Izabela Figiel; Evgeni Ponimaskin; Jakub Wlodarczyk; Jacek Jaworski; Grzegorz M. Wilczynski; Joanna Dzwonek

doi:10.1242/jcs.154542

CD44 regulates dendrite morphogenesis through Src tyrosine kinase-dependent positioning of the Golgi

Anna Skupien, Anna Konopka, Pawe I. Trzaskoma, Josephine Labus, Adam Gorlewicz, Lukasz Swiech, Matylda Babraj, Hubert Dolezyczek, Izabela Figiel, Evgeni Ponimaskin, Jakub Wlodarczyk, Jacek Jaworski, Grzegorz M. Wilczynski, Joanna Dzwonek

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

The acquisition of proper dendrite morphology is a crucial aspect of neuronal development towards the formation of a functional network. The role of the extracellular matrix and its cellular receptors in this process has remained enigmatic. We report that the CD44 adhesion molecule, the main hyaluronan receptor, is localized in dendrites and plays a crucial inhibitory role in dendritic tree arborization in vitro and in vivo. This novel function is exerted by the activation of Src tyrosine kinase, leading to the alteration of Golgi morphology. The mechanism operates during normal brain development, but its inhibition might have a protective influence on dendritic trees under toxic conditions, during which the silencing of CD44 expression prevents dendritic shortening induced by glutamate exposure. Overall, our results indicate a novel role for CD44 as an essential regulator of dendritic arbor complexity in both health and disease.

Original language	English
Pages (from-to)	5038-5051
Number of pages	14
Journal	Journal of Cell Science
Volume	127
Issue number	23
DOIs	https://doi.org/10.1242/jcs.154542
Publication status	Published - 1 Dec 2014
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2014. Published by The Company of Biologists Ltd.

Keywords

CD44
Dendritic arborization
Extracellular matrix
Golgi fragmentation
Src kinase

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Skupien, A., Konopka, A., Trzaskoma, P. I., Labus, J., Gorlewicz, A., Swiech, L., Babraj, M., Dolezyczek, H., Figiel, I., Ponimaskin, E., Wlodarczyk, J., Jaworski, J., Wilczynski, G. M., & Dzwonek, J. (2014). CD44 regulates dendrite morphogenesis through Src tyrosine kinase-dependent positioning of the Golgi. Journal of Cell Science, 127(23), 5038-5051. https://doi.org/10.1242/jcs.154542

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title = "CD44 regulates dendrite morphogenesis through Src tyrosine kinase-dependent positioning of the Golgi",

abstract = "The acquisition of proper dendrite morphology is a crucial aspect of neuronal development towards the formation of a functional network. The role of the extracellular matrix and its cellular receptors in this process has remained enigmatic. We report that the CD44 adhesion molecule, the main hyaluronan receptor, is localized in dendrites and plays a crucial inhibitory role in dendritic tree arborization in vitro and in vivo. This novel function is exerted by the activation of Src tyrosine kinase, leading to the alteration of Golgi morphology. The mechanism operates during normal brain development, but its inhibition might have a protective influence on dendritic trees under toxic conditions, during which the silencing of CD44 expression prevents dendritic shortening induced by glutamate exposure. Overall, our results indicate a novel role for CD44 as an essential regulator of dendritic arbor complexity in both health and disease.",

keywords = "CD44, Dendritic arborization, Extracellular matrix, Golgi fragmentation, Src kinase",

author = "Anna Skupien and Anna Konopka and Trzaskoma, {Pawe I.} and Josephine Labus and Adam Gorlewicz and Lukasz Swiech and Matylda Babraj and Hubert Dolezyczek and Izabela Figiel and Evgeni Ponimaskin and Jakub Wlodarczyk and Jacek Jaworski and Wilczynski, {Grzegorz M.} and Joanna Dzwonek",

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doi = "10.1242/jcs.154542",

language = "English",

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Skupien, A, Konopka, A, Trzaskoma, PI, Labus, J, Gorlewicz, A, Swiech, L, Babraj, M, Dolezyczek, H, Figiel, I, Ponimaskin, E, Wlodarczyk, J, Jaworski, J, Wilczynski, GM & Dzwonek, J 2014, 'CD44 regulates dendrite morphogenesis through Src tyrosine kinase-dependent positioning of the Golgi', Journal of Cell Science, vol. 127, no. 23, pp. 5038-5051. https://doi.org/10.1242/jcs.154542

TY - JOUR

T1 - CD44 regulates dendrite morphogenesis through Src tyrosine kinase-dependent positioning of the Golgi

AU - Skupien, Anna

AU - Konopka, Anna

AU - Trzaskoma, Pawe I.

AU - Labus, Josephine

AU - Gorlewicz, Adam

AU - Swiech, Lukasz

AU - Babraj, Matylda

AU - Dolezyczek, Hubert

AU - Figiel, Izabela

AU - Ponimaskin, Evgeni

AU - Wlodarczyk, Jakub

AU - Jaworski, Jacek

AU - Wilczynski, Grzegorz M.

AU - Dzwonek, Joanna

PY - 2014/12/1

Y1 - 2014/12/1

N2 - The acquisition of proper dendrite morphology is a crucial aspect of neuronal development towards the formation of a functional network. The role of the extracellular matrix and its cellular receptors in this process has remained enigmatic. We report that the CD44 adhesion molecule, the main hyaluronan receptor, is localized in dendrites and plays a crucial inhibitory role in dendritic tree arborization in vitro and in vivo. This novel function is exerted by the activation of Src tyrosine kinase, leading to the alteration of Golgi morphology. The mechanism operates during normal brain development, but its inhibition might have a protective influence on dendritic trees under toxic conditions, during which the silencing of CD44 expression prevents dendritic shortening induced by glutamate exposure. Overall, our results indicate a novel role for CD44 as an essential regulator of dendritic arbor complexity in both health and disease.

AB - The acquisition of proper dendrite morphology is a crucial aspect of neuronal development towards the formation of a functional network. The role of the extracellular matrix and its cellular receptors in this process has remained enigmatic. We report that the CD44 adhesion molecule, the main hyaluronan receptor, is localized in dendrites and plays a crucial inhibitory role in dendritic tree arborization in vitro and in vivo. This novel function is exerted by the activation of Src tyrosine kinase, leading to the alteration of Golgi morphology. The mechanism operates during normal brain development, but its inhibition might have a protective influence on dendritic trees under toxic conditions, during which the silencing of CD44 expression prevents dendritic shortening induced by glutamate exposure. Overall, our results indicate a novel role for CD44 as an essential regulator of dendritic arbor complexity in both health and disease.

KW - CD44

KW - Dendritic arborization

KW - Extracellular matrix

KW - Golgi fragmentation

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AN - SCOPUS:84919326437

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JO - Journal of Cell Science

JF - Journal of Cell Science

IS - 23

ER -

CD44 regulates dendrite morphogenesis through Src tyrosine kinase-dependent positioning of the Golgi

Abstract

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Keywords

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