cDNA cloning and characterization of the human UDP glucuronosyltransferase, UGT1A3

B. Mojarrabi, R. Butler, P. I. Mackenzie

Research output: Contribution to journalArticlepeer-review

102 Citations (Scopus)

Abstract

The cDNA encoding the UDP glucuronosyltransferase, UGT1A3, has been cloned and expressed in cell culture. The deduced amino acid sequence of the cDNA is 90% similar in sequence to that of a previously characterized form, UGT1A4 and to that of a third form, UGT1A5 whose function in unknown. UGT1A3, when expressed in COS cells has a relative molecular mass of 55 kDa and is active in the glucuronidation of estrone and 2-hydroxyestrone. Activity towards other polyhydroxylated estrogens including 4-hydroxyestrogen and estriol and its metabolites was not detected. UGT1A3 was also inactive towards androgens and their metabolites. The enzyme preferentially glucuronidated the N-hydroxy metabolite of 2-acetylaminofluorene and was more active towards the 6- and 12-hydroxylated metabolites of benzo[α]pyrene. RNA encoding UGT1A3 was detected in human liver and colon tissue.

Original languageEnglish
Pages (from-to)785-790
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume225
Issue number3
DOIs
Publication statusPublished - 23 Aug 1996
Externally publishedYes

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