TY - JOUR
T1 - cDNA cloning and characterization of the human UDP glucuronosyltransferase, UGT1A3
AU - Mojarrabi, B.
AU - Butler, R.
AU - Mackenzie, P. I.
PY - 1996/8/23
Y1 - 1996/8/23
N2 - The cDNA encoding the UDP glucuronosyltransferase, UGT1A3, has been cloned and expressed in cell culture. The deduced amino acid sequence of the cDNA is 90% similar in sequence to that of a previously characterized form, UGT1A4 and to that of a third form, UGT1A5 whose function in unknown. UGT1A3, when expressed in COS cells has a relative molecular mass of 55 kDa and is active in the glucuronidation of estrone and 2-hydroxyestrone. Activity towards other polyhydroxylated estrogens including 4-hydroxyestrogen and estriol and its metabolites was not detected. UGT1A3 was also inactive towards androgens and their metabolites. The enzyme preferentially glucuronidated the N-hydroxy metabolite of 2-acetylaminofluorene and was more active towards the 6- and 12-hydroxylated metabolites of benzo[α]pyrene. RNA encoding UGT1A3 was detected in human liver and colon tissue.
AB - The cDNA encoding the UDP glucuronosyltransferase, UGT1A3, has been cloned and expressed in cell culture. The deduced amino acid sequence of the cDNA is 90% similar in sequence to that of a previously characterized form, UGT1A4 and to that of a third form, UGT1A5 whose function in unknown. UGT1A3, when expressed in COS cells has a relative molecular mass of 55 kDa and is active in the glucuronidation of estrone and 2-hydroxyestrone. Activity towards other polyhydroxylated estrogens including 4-hydroxyestrogen and estriol and its metabolites was not detected. UGT1A3 was also inactive towards androgens and their metabolites. The enzyme preferentially glucuronidated the N-hydroxy metabolite of 2-acetylaminofluorene and was more active towards the 6- and 12-hydroxylated metabolites of benzo[α]pyrene. RNA encoding UGT1A3 was detected in human liver and colon tissue.
UR - http://www.scopus.com/inward/record.url?scp=0013588037&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.1251
DO - 10.1006/bbrc.1996.1251
M3 - Article
C2 - 8780690
AN - SCOPUS:0013588037
VL - 225
SP - 785
EP - 790
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -