TY - JOUR
T1 - cDNA cloning and expression of two new members of the human liver UDP-glucuronosyltransferase 2B subfamily
AU - Jin, C. J.
AU - Miners, J. O.
AU - Lillywhite, K. J.
AU - Mackenzie, P. I.
PY - 1993/7/15
Y1 - 1993/7/15
N2 - Two new UDP-glucuronosyltransferase cDNAs, designated UGT2B10 and UGT2B11, encoding 528 amino acid proteins were isolated from a human liver cDNA library. The deduced amino acid sequences of UGTs 2B10 and 2B11 share >76% sequence similarity with other known human liver UGT2B subfamily isoforms and <48% sequence similarity with UGT1 family proteins. COS-7 cells transfected with UGT 2B10 and 2B11 synthesized proteins with respective molecular masses of 49 kDa and 51 kDa. UGT2B11 expressed in COS-7 cells glucuronidated a number of polyhydroxylated estrogens (estriol, 4-hydroxyestrone and 2-hydroxyestriol) and xenobiotics (4-methylumbelliferone, 1-naphthol, 4-nitrophenol, 2-aminophenol, 4-hydroxybiphenyl and menthol). Despite the screening of more than forty potential substrates, glucuronidation activity was not observed for expressed UGT2B10.
AB - Two new UDP-glucuronosyltransferase cDNAs, designated UGT2B10 and UGT2B11, encoding 528 amino acid proteins were isolated from a human liver cDNA library. The deduced amino acid sequences of UGTs 2B10 and 2B11 share >76% sequence similarity with other known human liver UGT2B subfamily isoforms and <48% sequence similarity with UGT1 family proteins. COS-7 cells transfected with UGT 2B10 and 2B11 synthesized proteins with respective molecular masses of 49 kDa and 51 kDa. UGT2B11 expressed in COS-7 cells glucuronidated a number of polyhydroxylated estrogens (estriol, 4-hydroxyestrone and 2-hydroxyestriol) and xenobiotics (4-methylumbelliferone, 1-naphthol, 4-nitrophenol, 2-aminophenol, 4-hydroxybiphenyl and menthol). Despite the screening of more than forty potential substrates, glucuronidation activity was not observed for expressed UGT2B10.
UR - http://www.scopus.com/inward/record.url?scp=0027267719&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1993.1847
DO - 10.1006/bbrc.1993.1847
M3 - Article
AN - SCOPUS:0027267719
SN - 0006-291X
VL - 194
SP - 496
EP - 503
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -