TY - JOUR
T1 - Cell culture-Taqman PCR assay for evaluation of Cryptosporidium parvum disinfection
AU - Keegan, Alexandra R.
AU - Fanok, Stella
AU - Monis, Paul T.
AU - Saint, Christopher P.
PY - 2003/5/1
Y1 - 2003/5/1
N2 - Cryptosporidium parvum represents a challenge to the water industry and a threat to public health. In this study, we developed a cell culture-quantitative PCR assay to evaluate the inactivation of C. parvum with disinfectants. The assay was validated by using a range of disinfectants in common use in the water industry, including low-pressure UV light (LP-UV), ozone, mixed oxidants (MIOX), and chlorine. The assay was demonstrated to be reliable and sensitive, with a lower detection limit of a single infectious oocyst. Effective oocystinactivation was achieved (>2 log10 units) with LP-UV (20 mJ/cm2) or 2 mg of ozone/liter (for 10 min). MIOX and chlorine treatments of oocysts resulted in minimal effective disinfection, with <0.1 log10 unit being inactivated. These results demonstrate the inability of MIOX to inactivate Cryptosporidium. The assay is a valuable tool for the evaluation of disinfection systems for drinking water and recycled water.
AB - Cryptosporidium parvum represents a challenge to the water industry and a threat to public health. In this study, we developed a cell culture-quantitative PCR assay to evaluate the inactivation of C. parvum with disinfectants. The assay was validated by using a range of disinfectants in common use in the water industry, including low-pressure UV light (LP-UV), ozone, mixed oxidants (MIOX), and chlorine. The assay was demonstrated to be reliable and sensitive, with a lower detection limit of a single infectious oocyst. Effective oocystinactivation was achieved (>2 log10 units) with LP-UV (20 mJ/cm2) or 2 mg of ozone/liter (for 10 min). MIOX and chlorine treatments of oocysts resulted in minimal effective disinfection, with <0.1 log10 unit being inactivated. These results demonstrate the inability of MIOX to inactivate Cryptosporidium. The assay is a valuable tool for the evaluation of disinfection systems for drinking water and recycled water.
UR - http://www.scopus.com/inward/record.url?scp=0037545834&partnerID=8YFLogxK
U2 - 10.1128/AEM.69.5.2505-2511.2003
DO - 10.1128/AEM.69.5.2505-2511.2003
M3 - Article
C2 - 12732515
AN - SCOPUS:0037545834
SN - 0099-2240
VL - 69
SP - 2505
EP - 2511
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 5
ER -
