Cell-Free Synthesis of a Functional Membrane Transporter into a Tethered Bilayer Lipid Membrane

Julius Zieleniecki, Yagnesh Nagarajan, Shane Waters, Jay Rongala, Vanessa Thompson, Maria Hrmova, Ingo Koper

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    Eukaryotic cell-free synthesis was used to incorporate the large and complex multispan plant membrane transporter Bot1 in a functional form into a tethered bilayer lipid membrane. The electrical properties of the protein-functionalized tethered bilayer were measured using electrochemical impedance spectroscopy and revealed a pH-dependent transport of borate ions through the protein. The efficacy of the protein synthesis has been evaluated using immunoblot analysis. (Figure Presented).

    Original languageEnglish
    Pages (from-to)2445-2449
    Number of pages5
    JournalLangmuir
    Volume32
    Issue number10
    DOIs
    Publication statusPublished - 15 Mar 2016

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