The cell kinetic parameters of K‐562 leukemia cells were studied using microwell cultures in which growth was initiated from a single cell. Total population growth was studied by direct enumeration, 3H‐thymidine labelling, and flow cytometry. Clonogenic cell growth was studied by replating and 3H‐thymidine suicide. In 7‐day clones of K‐562 cells, durations of the total cell cycle, G1, S, G2, and M phases were 20.8 h, 3.5 h, 12.9 h, 3.3 h, and 1.1 h, respectively; the growth fraction was 0.92 and the cell loss factor was 0.084. Study of colony‐forming cells by replating indicated that clonogenic cells comprised 40% of total cells. 3H‐Thymidine suicide showed that cell‐cycle duration for these cells was 22.5 h and that S‐phase duration was 11.7 h.
- Cell kinetics
- Colony‐forming cells