Cell-to-cell transport via the lumen of the endoplasmic reticulum

Deborah Barton, Louise Cole, David Collings, Danny Liu, Penelope Smith, David Day, Robyn Overall

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    34 Citations (Scopus)

    Abstract

    Summary Plasmodesmata are plasma membrane-lined channels through which cytoplasmic molecules move from cell-to-cell in plants. Most plasmodesmata contain a desmotubule, a central tube of endoplasmic reticulum (ER), that connects the ER of adjacent cells. Here we demonstrate that molecules of up to 10.4 kDa in size can move between the ER lumen of neighbouring leaf trichome or epidermal cells via the desmotubule lumen. Fluorescent molecules of up to 10 kDa, microinjected into the ER of Nicotiana trichome cells, consistently moved into the ER and nuclei of neighbouring trichome cells. This movement occurred more rapidly than movement via the cytoplasmic pathway. A fluorescent 3-kDa dextran microinjected into the ER of a basal trichome cell moved into the ER and nuclei of epidermal cells across a barrier to cytoplasmic movement. We constructed a 10.4-kDa recombinant ER-lumenal reporter protein (LRP) from a fragment of the endogenous ER-lumenal binding protein AtBIP1. Following transient expression of the LRP in the ER of Tradescantia leaf epidermal cells, it often moved into the nuclear envelopes of neighbouring cells. However, green fluorescent protein targeted to the ER lumen (ER-GFP) did not move from cell to cell. We propose that the ER lumen of plant cells is continuous with that of their neighbours, and allows movement of small ER-lumenal molecules between cells.

    Original languageEnglish
    Pages (from-to)806-817
    Number of pages12
    JournalThe Plant Journal
    Volume66
    Issue number5
    DOIs
    Publication statusPublished - Jun 2011

    Keywords

    • endoplasmic reticulum
    • ER-GFP
    • intercellular transport
    • microinjection
    • tobacco
    • trichome

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  • Cite this

    Barton, D., Cole, L., Collings, D., Liu, D., Smith, P., Day, D., & Overall, R. (2011). Cell-to-cell transport via the lumen of the endoplasmic reticulum. The Plant Journal, 66(5), 806-817. https://doi.org/10.1111/j.1365-313X.2011.04545.x