TY - JOUR
T1 - Characterisation of major histocompatibility complex class I transcripts in an Australian dragon lizard
AU - Hacking, Jessica
AU - Bertozzi, Terry
AU - Moussalli, Adnan
AU - Bradford, Tessa
AU - Gardner, Mike
PY - 2018/7
Y1 - 2018/7
N2 - Characterisation of squamate major histocompatibility complex (MHC) genes has lagged behind other taxonomic groups. MHC genes encode cell-surface glycoproteins that present self- and pathogen-derived peptides to T cells and play a critical role in pathogen recognition. Here we characterise MHC class I transcripts for an agamid lizard (Ctenophorus decresii) and investigate the evolution of MHC class I in Iguanian lizards. An iterative assembly strategy was used to identify six full-length C. decresii MHC class I transcripts, which were validated as likely to encode classical class I MHC molecules. Evidence for exon shuffling recombination was uncovered for C. decresii transcripts and Bayesian phylogenetic analysis of Iguanian MHC class I sequences revealed a pattern expected under a birth-and-death mode of evolution. This work provides a stepping stone towards further research on the agamid MHC class I region.
AB - Characterisation of squamate major histocompatibility complex (MHC) genes has lagged behind other taxonomic groups. MHC genes encode cell-surface glycoproteins that present self- and pathogen-derived peptides to T cells and play a critical role in pathogen recognition. Here we characterise MHC class I transcripts for an agamid lizard (Ctenophorus decresii) and investigate the evolution of MHC class I in Iguanian lizards. An iterative assembly strategy was used to identify six full-length C. decresii MHC class I transcripts, which were validated as likely to encode classical class I MHC molecules. Evidence for exon shuffling recombination was uncovered for C. decresii transcripts and Bayesian phylogenetic analysis of Iguanian MHC class I sequences revealed a pattern expected under a birth-and-death mode of evolution. This work provides a stepping stone towards further research on the agamid MHC class I region.
KW - Agamidae
KW - Ctenophorus decresii
KW - Iguania
KW - MHC class I evolution
KW - Transcriptome assembly
UR - http://www.scopus.com/inward/record.url?scp=85042231084&partnerID=8YFLogxK
U2 - 10.1016/j.dci.2018.02.012
DO - 10.1016/j.dci.2018.02.012
M3 - Article
VL - 84
SP - 164
EP - 171
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
SN - 0145-305X
ER -