Characterisation of microsatellite markers for the granite endemic Kunzea pulchella (Lindl.) A. S. George (Myrtaceae) identified using next generation sequencing

S.-L. Tapper, Margaret Byrne, Michael Gardner

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    A set of 11 polymorphic microsatellite loci were developed for Kunzea pulchella, a plant species endemic to granite outcrops in Western Australia. Genomic sequences were obtained from next generation (454) sequencing. A total of 20 microsatellite markers were then chosen for amplification and genotyping trials in individuals across the species range. Eleven of these loci were selected for analysis based on amplification and genotyping success. All were polymorphic with 4-10 alleles per locus (mean = 5. 9). Expected and observed heterozygosity ranged from 0. 29 to 0. 92 averaging 0. 606 and 0. 690 respectively. Loci were in Hardy-Weinberg equilibrium (P < 0. 05) except for two which showed evidence for null alleles. Linkage disequilibrium was evident in three loci pair combinations. These polymorphic microsatellite markers will be valuable for analysis of population genetic structure and connectivity in K. pulchella.

    Original languageEnglish
    Pages (from-to)129-131
    Number of pages3
    JournalConservation Genetics Resources
    Volume5
    Issue number1
    DOIs
    Publication statusPublished - 2013

    Keywords

    • Genetic diversity
    • Granite outcrop
    • Refugia
    • Western Australia

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