TY - JOUR
T1 - Characterisation of novel and rare Y-chromosome short tandem repeat alleles in self-declared South Australian Aboriginal database
AU - Collins, Tegan
AU - Ottens, Renee
AU - Ballantyne, Kaye
AU - Nagle, Nano
AU - Henry, Julianne
AU - Taylor, Duncan
AU - Gardner, Michael
AU - Fitch, Alison
AU - Goodman, Amanda
AU - van Oorschot, Roland
AU - Mitchell, R.
AU - Linacre, Adrian
PY - 2014/1
Y1 - 2014/1
N2 - Y-chromosome short tandem repeats (Y-STRs) are used in forensic science laboratories all over the world, as their application is wide and often vital in solving casework. Analysis of an in-house database of South Australian self-declared Aboriginal males held by Forensic Science South Australia (FSSA) using the Applied Biosystem's AmpFℓSTR® Yfiler™ PCR Amplification Kit revealed 43 variant Y-STR alleles at 6 of the 17 loci. All variant alleles were sequenced to determine the exact repeat structure for each. As a high level of admixture has previously been found within the SA Aboriginal database, samples were haplogrouped using Y-SNPs to determine their likely geographical origin. Although a number of variant alleles were associated with non-Aboriginal Y-haplogroups, a high frequency was observed within the Australian K-M9 lineage. Detailed knowledge of these variant alleles may have further application in the development of new DNA markers for identification purposes, and in population and evolutionary studies of Australian Aborigines.
AB - Y-chromosome short tandem repeats (Y-STRs) are used in forensic science laboratories all over the world, as their application is wide and often vital in solving casework. Analysis of an in-house database of South Australian self-declared Aboriginal males held by Forensic Science South Australia (FSSA) using the Applied Biosystem's AmpFℓSTR® Yfiler™ PCR Amplification Kit revealed 43 variant Y-STR alleles at 6 of the 17 loci. All variant alleles were sequenced to determine the exact repeat structure for each. As a high level of admixture has previously been found within the SA Aboriginal database, samples were haplogrouped using Y-SNPs to determine their likely geographical origin. Although a number of variant alleles were associated with non-Aboriginal Y-haplogroups, a high frequency was observed within the Australian K-M9 lineage. Detailed knowledge of these variant alleles may have further application in the development of new DNA markers for identification purposes, and in population and evolutionary studies of Australian Aborigines.
KW - Australian Aboriginal males
KW - Forensic science
KW - Human identification
KW - Y-chromosome
KW - Y-STR
UR - http://www.scopus.com/inward/record.url?scp=84891825988&partnerID=8YFLogxK
U2 - 10.1007/s00414-013-0911-y
DO - 10.1007/s00414-013-0911-y
M3 - Article
VL - 128
SP - 27
EP - 31
JO - International Journal of Legal Medicine
JF - International Journal of Legal Medicine
SN - 0937-9827
IS - 1
ER -