Characterization and in vitro translation of Toxoplasma gondii ribonucleic acid

Alan M. Johnson, P. J. McDonald, Susana Illana

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)


    RNA was extracted from purified tachyzoites of Toxoplasma gondii (RH strain) by sequential centrifugation in guanidine hydrochloride, urea, and lithium chloride. The subunits of the RNA were characterized by denaturing and non-denaturing electrophoresis in agarose gels. Poly(A)+-RNA, purified by oligo(dT)-cellulose affinity chromatography, was translated in a rabbit reticulocyte lysate assay and the products were immunoprecipitated with an experimentally infected mouse serum and a naturally infected human serum. After sodium dodecyl sulphate-polyacrylamide gel electrophoresis, fluorography of the polypeptides confirmed that the mRNA translated specific parasite antigens.

    Original languageEnglish
    Pages (from-to)313-320
    Number of pages8
    JournalMolecular and Biochemical Parasitology
    Issue number3
    Publication statusPublished - Mar 1986


    • Antigenic polypeptides
    • Apparent relative molecular mass
    • Immunoprecipitation
    • In vitro translation
    • mRNA
    • rRNA
    • T. gondii antibody
    • Toxoplasma gondii


    Dive into the research topics of 'Characterization and in vitro translation of Toxoplasma gondii ribonucleic acid'. Together they form a unique fingerprint.

    Cite this