Characterization and in vitro translation of Toxoplasma gondii ribonucleic acid

Alan M. Johnson, P. J. McDonald, Susana Illana

    Research output: Contribution to journalArticlepeer-review

    20 Citations (Scopus)

    Abstract

    RNA was extracted from purified tachyzoites of Toxoplasma gondii (RH strain) by sequential centrifugation in guanidine hydrochloride, urea, and lithium chloride. The subunits of the RNA were characterized by denaturing and non-denaturing electrophoresis in agarose gels. Poly(A)+-RNA, purified by oligo(dT)-cellulose affinity chromatography, was translated in a rabbit reticulocyte lysate assay and the products were immunoprecipitated with an experimentally infected mouse serum and a naturally infected human serum. After sodium dodecyl sulphate-polyacrylamide gel electrophoresis, fluorography of the polypeptides confirmed that the mRNA translated specific parasite antigens.

    Original languageEnglish
    Pages (from-to)313-320
    Number of pages8
    JournalMolecular and Biochemical Parasitology
    Volume18
    Issue number3
    DOIs
    Publication statusPublished - Mar 1986

    Keywords

    • Antigenic polypeptides
    • Apparent relative molecular mass
    • Immunoprecipitation
    • In vitro translation
    • mRNA
    • rRNA
    • T. gondii antibody
    • Toxoplasma gondii

    Fingerprint Dive into the research topics of 'Characterization and in vitro translation of <i>Toxoplasma gondii</i> ribonucleic acid'. Together they form a unique fingerprint.

    Cite this