Characterization and in vitro translation of Toxoplasma gondii ribonucleic acid

Alan M. Johnson; P. J. McDonald; Susana Illana

doi:10.1016/0166-6851(86)90088-5

Characterization and in vitro translation of Toxoplasma gondii ribonucleic acid

Alan M. Johnson, P. J. McDonald, Susana Illana

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22 Citations (Scopus)

Abstract

RNA was extracted from purified tachyzoites of Toxoplasma gondii (RH strain) by sequential centrifugation in guanidine hydrochloride, urea, and lithium chloride. The subunits of the RNA were characterized by denaturing and non-denaturing electrophoresis in agarose gels. Poly(A)⁺-RNA, purified by oligo(dT)-cellulose affinity chromatography, was translated in a rabbit reticulocyte lysate assay and the products were immunoprecipitated with an experimentally infected mouse serum and a naturally infected human serum. After sodium dodecyl sulphate-polyacrylamide gel electrophoresis, fluorography of the polypeptides confirmed that the mRNA translated specific parasite antigens.

Original language	English
Pages (from-to)	313-320
Number of pages	8
Journal	Molecular and Biochemical Parasitology
Volume	18
Issue number	3
DOIs	https://doi.org/10.1016/0166-6851(86)90088-5
Publication status	Published - Mar 1986

Keywords

Antigenic polypeptides
Apparent relative molecular mass
Immunoprecipitation
In vitro translation
mRNA
rRNA
T. gondii antibody
Toxoplasma gondii

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title = "Characterization and in vitro translation of Toxoplasma gondii ribonucleic acid",

abstract = "RNA was extracted from purified tachyzoites of Toxoplasma gondii (RH strain) by sequential centrifugation in guanidine hydrochloride, urea, and lithium chloride. The subunits of the RNA were characterized by denaturing and non-denaturing electrophoresis in agarose gels. Poly(A)+-RNA, purified by oligo(dT)-cellulose affinity chromatography, was translated in a rabbit reticulocyte lysate assay and the products were immunoprecipitated with an experimentally infected mouse serum and a naturally infected human serum. After sodium dodecyl sulphate-polyacrylamide gel electrophoresis, fluorography of the polypeptides confirmed that the mRNA translated specific parasite antigens.",

keywords = "Antigenic polypeptides, Apparent relative molecular mass, Immunoprecipitation, In vitro translation, mRNA, rRNA, T. gondii antibody, Toxoplasma gondii",

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T1 - Characterization and in vitro translation of Toxoplasma gondii ribonucleic acid

AU - Johnson, Alan M.

AU - McDonald, P. J.

AU - Illana, Susana

PY - 1986/3

Y1 - 1986/3

N2 - RNA was extracted from purified tachyzoites of Toxoplasma gondii (RH strain) by sequential centrifugation in guanidine hydrochloride, urea, and lithium chloride. The subunits of the RNA were characterized by denaturing and non-denaturing electrophoresis in agarose gels. Poly(A)+-RNA, purified by oligo(dT)-cellulose affinity chromatography, was translated in a rabbit reticulocyte lysate assay and the products were immunoprecipitated with an experimentally infected mouse serum and a naturally infected human serum. After sodium dodecyl sulphate-polyacrylamide gel electrophoresis, fluorography of the polypeptides confirmed that the mRNA translated specific parasite antigens.

AB - RNA was extracted from purified tachyzoites of Toxoplasma gondii (RH strain) by sequential centrifugation in guanidine hydrochloride, urea, and lithium chloride. The subunits of the RNA were characterized by denaturing and non-denaturing electrophoresis in agarose gels. Poly(A)+-RNA, purified by oligo(dT)-cellulose affinity chromatography, was translated in a rabbit reticulocyte lysate assay and the products were immunoprecipitated with an experimentally infected mouse serum and a naturally infected human serum. After sodium dodecyl sulphate-polyacrylamide gel electrophoresis, fluorography of the polypeptides confirmed that the mRNA translated specific parasite antigens.

KW - Antigenic polypeptides

KW - Apparent relative molecular mass

KW - Immunoprecipitation

KW - In vitro translation

KW - mRNA

KW - rRNA

KW - T. gondii antibody

KW - Toxoplasma gondii

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JO - Molecular and Biochemical Parasitology

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Characterization and in vitro translation of Toxoplasma gondii ribonucleic acid

Abstract

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