CHARACTERIZATION AND REGULATION OF RAT LIVER UDP GLUCURONOSYLTRANSFERASES

P. I. Mackenzie, N. Roy Chowdhury, J. Roy Chowdhury

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

1. The use of DNA recombinant techniques to study UDP glucuronosyltrans‐ferases is reviewed. 2. The cloning and sequencing of the cDNAs to five forms of UDP glucuronosyl‐transferase demonstrated that these proteins are encoded by a superfamily of genes. 3. The substrate specificities of four isozymes have been analysed by expression of their corresponding cDNAs in cell culture. 4. Analysis of liver mRNA levels using radiolabelled DNA probes demonstrated that one isozyme, UDPGTr‐2, is elevated by phenobarbital whereas a second isozyme, 4‐nitrophenol GT is elevated by 3‐methylcholanthrene. The levels of other isozymes are unaffected by these two prototypic inducers of drug metabolizing enzymes. 5. The level of 4‐nitrophenol GT mRNA is elevated 10–15‐fold in hyperplastic nodules in livers of rats treated with 2‐acetylaminofluorene. Similar increases were not observed for mRNAs encoding other forms of UDP glucuronosyltransferase.

Original languageEnglish
Pages (from-to)501-504
Number of pages4
JournalClinical and Experimental Pharmacology and Physiology
Volume16
Issue number6
DOIs
Publication statusPublished - Jun 1989
Externally publishedYes

Keywords

  • carcinogenesis
  • isozymes
  • mRNA regulation
  • substrate specificity
  • UDP glucuronosyltransferase

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