Characterization of a novel carbonic anhydrase from freshwater pearl mussel Hyriopsis cumingii and the expression profile of its transcript in response to environmental conditions

Gang Ren; Yan Wang; Jianguang Qin; Jinyu Tang; Xiaofei Zheng; Youming Li

doi:10.1016/j.gene.2014.05.039

Characterization of a novel carbonic anhydrase from freshwater pearl mussel Hyriopsis cumingii and the expression profile of its transcript in response to environmental conditions

Gang Ren, Yan Wang, Jianguang Qin, Jinyu Tang, Xiaofei Zheng, Youming Li

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Gene encoding for α-carbonic anhydrases (α-CAs) and their functions in fundamental metabolism and biomineralization are widely identified in mollusks. However, the transcriptional regulation of α-CA genes in response to various environmental conditions remains unknown. In the present study, we characterized a cDNA encoding for an α-CA (HcCA) from the freshwater pearl mussel Hyriopsis cumingii. The spatial and temporal expression patterns of HcCA indicate that this gene is mainly expressed in the mantle of juvenile mussels. The expression profile of HcCA under various environmental conditions reveals that the transcription of HcCA is significantly regulated by Ca²⁺ concentration, water temperature, pH and air exposure. Our results suggest that HcCA is a crucial target gene by which the external environmental conditions affecting shell growth and pH homeostasis of H. cumingii. 2014 Elsevier B.V.

Original language	English
Pages (from-to)	56-62
Number of pages	7
Journal	Gene
Volume	546
Issue number	1
DOIs	https://doi.org/10.1016/j.gene.2014.05.039
Publication status	Published - 1 Aug 2014

Keywords

Carbonic anhydrase
Expression
Hyriopsis cumingii
PH homeostasis
Shell growth

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10.1016/j.gene.2014.05.039

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title = "Characterization of a novel carbonic anhydrase from freshwater pearl mussel Hyriopsis cumingii and the expression profile of its transcript in response to environmental conditions",

abstract = "Gene encoding for α-carbonic anhydrases (α-CAs) and their functions in fundamental metabolism and biomineralization are widely identified in mollusks. However, the transcriptional regulation of α-CA genes in response to various environmental conditions remains unknown. In the present study, we characterized a cDNA encoding for an α-CA (HcCA) from the freshwater pearl mussel Hyriopsis cumingii. The spatial and temporal expression patterns of HcCA indicate that this gene is mainly expressed in the mantle of juvenile mussels. The expression profile of HcCA under various environmental conditions reveals that the transcription of HcCA is significantly regulated by Ca2+ concentration, water temperature, pH and air exposure. Our results suggest that HcCA is a crucial target gene by which the external environmental conditions affecting shell growth and pH homeostasis of H. cumingii. 2014 Elsevier B.V.",

keywords = "Carbonic anhydrase, Expression, Hyriopsis cumingii, PH homeostasis, Shell growth",

author = "Gang Ren and Yan Wang and Jianguang Qin and Jinyu Tang and Xiaofei Zheng and Youming Li",

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T1 - Characterization of a novel carbonic anhydrase from freshwater pearl mussel Hyriopsis cumingii and the expression profile of its transcript in response to environmental conditions

AU - Ren, Gang

AU - Wang, Yan

AU - Qin, Jianguang

AU - Tang, Jinyu

AU - Zheng, Xiaofei

AU - Li, Youming

PY - 2014/8/1

Y1 - 2014/8/1

N2 - Gene encoding for α-carbonic anhydrases (α-CAs) and their functions in fundamental metabolism and biomineralization are widely identified in mollusks. However, the transcriptional regulation of α-CA genes in response to various environmental conditions remains unknown. In the present study, we characterized a cDNA encoding for an α-CA (HcCA) from the freshwater pearl mussel Hyriopsis cumingii. The spatial and temporal expression patterns of HcCA indicate that this gene is mainly expressed in the mantle of juvenile mussels. The expression profile of HcCA under various environmental conditions reveals that the transcription of HcCA is significantly regulated by Ca2+ concentration, water temperature, pH and air exposure. Our results suggest that HcCA is a crucial target gene by which the external environmental conditions affecting shell growth and pH homeostasis of H. cumingii. 2014 Elsevier B.V.

AB - Gene encoding for α-carbonic anhydrases (α-CAs) and their functions in fundamental metabolism and biomineralization are widely identified in mollusks. However, the transcriptional regulation of α-CA genes in response to various environmental conditions remains unknown. In the present study, we characterized a cDNA encoding for an α-CA (HcCA) from the freshwater pearl mussel Hyriopsis cumingii. The spatial and temporal expression patterns of HcCA indicate that this gene is mainly expressed in the mantle of juvenile mussels. The expression profile of HcCA under various environmental conditions reveals that the transcription of HcCA is significantly regulated by Ca2+ concentration, water temperature, pH and air exposure. Our results suggest that HcCA is a crucial target gene by which the external environmental conditions affecting shell growth and pH homeostasis of H. cumingii. 2014 Elsevier B.V.

KW - Carbonic anhydrase

KW - Expression

KW - Hyriopsis cumingii

KW - PH homeostasis

KW - Shell growth

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DO - 10.1016/j.gene.2014.05.039

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JO - Gene

JF - Gene

IS - 1

ER -

Characterization of a novel carbonic anhydrase from freshwater pearl mussel Hyriopsis cumingii and the expression profile of its transcript in response to environmental conditions

Abstract

Keywords

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