Characterization of impedance biosensing performance of single and nanopore arrays of anodic porous alumina fabricated by focused ion beam (FIB) milling

Krishna Kant, Craig Priest, Joseph Shapter, Dusan Losic

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Nanoporous alumina (NPA) arrays prepared by electrochemical anodization have been attractive platform for application in molecular separations, biosensing, drug delivery and fuel cells due to their highly ordered pore structures, controllable pore dimensions and unique physical, optical, transport and electrochemical properties. However, there is still a lack of knowledge about the influence of the pore dimensions, shape and surface area on these properties. Hence the aim of this work is to use a focused ion beam (FIB) to mill a barrier layer of NPA to open a either single nanopore, several nanopores and 1 μm and 20 μm square areas of nanopore arrays to explore their electrochemical properties and biosensing performances. The internal surface of opened NPA nanopores was modified by covalent attachment of streptavidin and used for impedance label-free biosensing. The biosensor performance of this antibody modified nanopore biosensing platform was evaluated using various concentrations of biotin as a model analyte. Electrochemical impedance spectrometric (EIS) measurements of pore resistivity were carried out for different patterns of nanopore arrays. The results showed that small areas or number of pores provide better biosensing performance compared to large nanopore arrays.

    Original languageEnglish
    Pages (from-to)225-231
    Number of pages7
    JournalElectrochimica Acta
    Volume139
    DOIs
    Publication statusPublished - 1 Sep 2014

    Keywords

    • Electrochemical Impedance Spectroscopy (EIS)
    • Focused Ion beam milling
    • Impedance biosensing
    • Nanoporous alumina

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