Characterization of myenteric interneurons with somatostatin immunoreactivity in the guinea-pig small intestine

Z. M. Song, S. J.H. Brookes, G. A. Ramsay, M. Costa

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    51 Citations (Scopus)

    Abstract

    The projections, connections, morphology and electrophysiological features of the myenteric interneurons with somatostatin immunoreactivity in the guinea-pig small intestine have been established using retrograde tracing, immunohistochemistry, confocal microscopy and intracellular recording. After application of the fluorescent dye. 1,1' -didodecyl- 3,3,3',3'-tetramethyl indocarbocyanine perchlorate (DiI), to the myenteric plexus, up to 900 nerve cell bodies were labelled in each preparation. Somatostatin-immunoreactive neurons accounted for 13% of all retrogradely labelled cells and were located up to 70 mm orally. When DiI was applied to the submucous ganglia, many myenteric neurons were labelled and 8% of all retrogradely labelled cells were somatostatin immunoreactive and were located up to 60 mm oral to the DiI application sites. These neurons had ovoid cell bodies, a single axon, several long filamentous dendrites and received close contacts from 40-200 somatostatin-immunoreactive varicosities. Intracellular recordings revealed that these cells had features of both S (i.e. with Synaptic inputs) and AH (i.e. neurons with After Hyperpolarization) cells, receiving fast excitatory synaptic inputs, having characteristic 'sag' in their response to hyperpolarizing current pulses and sometimes a long afterhyperpolarization following soma action potentials. It is concluded that somatostatin-immunoreactive neurons have distinct electrophysiological features and form very long anally directed interneuronal chains that connect with both myenteric and submucous neurons.

    Original languageEnglish
    Pages (from-to)907-923
    Number of pages17
    JournalNeuroscience
    Volume80
    Issue number3
    DOIs
    Publication statusPublished - 28 Jul 1997

    Keywords

    • Electrophysiology
    • Guinea- pig
    • Myenteric plexus
    • Small intestine
    • Somatostatin
    • Submucous plexus

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