Characterization of photobiological hydrogen production by marine green algae platymonas subcordiformis

The hydrogenase activity of the marine green algae Platymonas subcordiformis at the late growth phase was the highest. The characterizations of photobiological hydrogen production for algae by sulfur element deprived from medium and treated with uncoupler CCCP were investigated. The sulfur element couldn't effectively regulate PSII photochemical activity, therefore it couldn't produce any hydrogen by sulfur-deprived during direct light illumination because hydrogenase couldn't expression. When algae treated with 5, 10, 15 and 20 μmol/L CCCP after 32 hours of dark anaerobic incubation of 32 hours, the PSII photochemical activity was obviously repressed, and the sealed system hold anaerobic condition, which could photoproduce 21.8, 23.0, 22.5 and 16.0 μmol/mg Chl, respectively. Besides, appropriate concentration CCCP could regulate algae hydrogen production under direct light illumination, but the quantity and rate of hydrogen production were lower than that of the former.