Characterization of reverse-engineered anti-PF4 stereotypic antibodies derived from serum of patients with VITT

Jing Jing Wang; Marijn van der Neut Kolfschoten; Lucy Rutten; Bridie Armour; Chee Wee Tan; Tim Chataway; Rinke Bos; Annemart Koornneef; Pravien Abeywickrema; Rick Kapur; Leendert Porcelijn; Midia Khalifa; Ava Sadi; Pascale Bouchier; Eleni Kourkouta; Aditya Perkasa; Ted Kwaks; Roland Zahn; Laura Solforosi; Tom P Gordon

doi:10.1182/blood.2023021307

Characterization of reverse-engineered anti-PF4 stereotypic antibodies derived from serum of patients with VITT

Jing Jing Wang, Marijn van der Neut Kolfschoten, Lucy Rutten, Bridie Armour, Chee Wee Tan, Tim Chataway, Rinke Bos, Annemart Koornneef, Pravien Abeywickrema, Rick Kapur, Leendert Porcelijn, Midia Khalifa, Ava Sadi, Pascale Bouchier, Eleni Kourkouta, Aditya Perkasa, Ted Kwaks, Roland Zahn, Laura Solforosi, Tom P Gordon

College of Medicine and Public Health

Research output: Contribution to journal › Letter › peer-review

3 Citations (Scopus)

Abstract

ABSTRACT This study presents a novel reverse-engineering approach to generate recombinant anti-PF4 antibodies from VITT-patient sera. These antibodies recapitulate functional effects of original serum anti-PF4 clonotypes, identify key PF4 binding residues and hold promise as valuable tools to investigate VITT etiology.

Original language	English
Pages (from-to)	370-374
Number of pages	5
Journal	Blood
Volume	143
Issue number	4
DOIs	https://doi.org/10.1182/blood.2023021307
Publication status	Published - 25 Jan 2024

Keywords

VITT
Anti-PF4
antibodies
Serum

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10.1182/blood.2023021307Licence: In Copyright

https://www.sciencedirect.com/science/article/pii/S0006497123143990

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Wang, J. J., van der Neut Kolfschoten, M., Rutten, L., Armour, B., Tan, C. W., Chataway, T., Bos, R., Koornneef, A., Abeywickrema, P., Kapur, R., Porcelijn, L., Khalifa, M., Sadi, A., Bouchier, P., Kourkouta, E., Perkasa, A., Kwaks, T., Zahn, R., Solforosi, L., & Gordon, T. P. (2024). Characterization of reverse-engineered anti-PF4 stereotypic antibodies derived from serum of patients with VITT. Blood, 143(4), 370-374. https://doi.org/10.1182/blood.2023021307

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title = "Characterization of reverse-engineered anti-PF4 stereotypic antibodies derived from serum of patients with VITT",

abstract = "ABSTRACT This study presents a novel reverse-engineering approach to generate recombinant anti-PF4 antibodies from VITT-patient sera. These antibodies recapitulate functional effects of original serum anti-PF4 clonotypes, identify key PF4 binding residues and hold promise as valuable tools to investigate VITT etiology.",

keywords = "VITT, Anti-PF4, antibodies, Serum",

author = "Wang, {Jing Jing} and {van der Neut Kolfschoten}, Marijn and Lucy Rutten and Bridie Armour and Tan, {Chee Wee} and Tim Chataway and Rinke Bos and Annemart Koornneef and Pravien Abeywickrema and Rick Kapur and Leendert Porcelijn and Midia Khalifa and Ava Sadi and Pascale Bouchier and Eleni Kourkouta and Aditya Perkasa and Ted Kwaks and Roland Zahn and Laura Solforosi and Gordon, {Tom P}",

year = "2024",

month = jan,

day = "25",

doi = "10.1182/blood.2023021307",

language = "English",

volume = "143",

pages = "370--374",

journal = "Blood",

issn = "0006-4971",

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Wang, JJ, van der Neut Kolfschoten, M, Rutten, L, Armour, B, Tan, CW, Chataway, T, Bos, R, Koornneef, A, Abeywickrema, P, Kapur, R, Porcelijn, L, Khalifa, M, Sadi, A, Bouchier, P, Kourkouta, E, Perkasa, A, Kwaks, T, Zahn, R, Solforosi, L & Gordon, TP 2024, 'Characterization of reverse-engineered anti-PF4 stereotypic antibodies derived from serum of patients with VITT', Blood, vol. 143, no. 4, pp. 370-374. https://doi.org/10.1182/blood.2023021307

TY - JOUR

T1 - Characterization of reverse-engineered anti-PF4 stereotypic antibodies derived from serum of patients with VITT

AU - Wang, Jing Jing

AU - van der Neut Kolfschoten, Marijn

AU - Rutten, Lucy

AU - Armour, Bridie

AU - Tan, Chee Wee

AU - Chataway, Tim

AU - Bos, Rinke

AU - Koornneef, Annemart

AU - Abeywickrema, Pravien

AU - Kapur, Rick

AU - Porcelijn, Leendert

AU - Khalifa, Midia

AU - Sadi, Ava

AU - Bouchier, Pascale

AU - Kourkouta, Eleni

AU - Perkasa, Aditya

AU - Kwaks, Ted

AU - Zahn, Roland

AU - Solforosi, Laura

AU - Gordon, Tom P

PY - 2024/1/25

Y1 - 2024/1/25

N2 - ABSTRACT This study presents a novel reverse-engineering approach to generate recombinant anti-PF4 antibodies from VITT-patient sera. These antibodies recapitulate functional effects of original serum anti-PF4 clonotypes, identify key PF4 binding residues and hold promise as valuable tools to investigate VITT etiology.

AB - ABSTRACT This study presents a novel reverse-engineering approach to generate recombinant anti-PF4 antibodies from VITT-patient sera. These antibodies recapitulate functional effects of original serum anti-PF4 clonotypes, identify key PF4 binding residues and hold promise as valuable tools to investigate VITT etiology.

KW - VITT

KW - Anti-PF4

KW - antibodies

KW - Serum

UR - http://www.scopus.com/inward/record.url?scp=85179466989&partnerID=8YFLogxK

U2 - 10.1182/blood.2023021307

DO - 10.1182/blood.2023021307

M3 - Letter

SN - 0006-4971

VL - 143

SP - 370

EP - 374

JO - Blood

JF - Blood

IS - 4

ER -

Characterization of reverse-engineered anti-PF4 stereotypic antibodies derived from serum of patients with VITT

Abstract

Keywords

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