Chloride analysis of botanical samples by ICP-OES

Matthew Wheal, Lyndon Palmer

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    23 Citations (Scopus)


    A unique method for chloride analysis of botanical samples by axially viewed ICP-OES with a modified optical system is described. Calibration on the most sensitive wavelength (134.7 nm) between 1 and 200 mg Cl L-1 yielded a curvilinear relationship (R2 = 1.000) across three orders of magnitude. The instrumental limits of detection in solution of 0.041 mg L-1 and background equivalent concentration of 5.29 mg L-1 are similar to those of other methods using pneumatic nebulisers. Matrix matching of calibration standards with respect to nitric acid concentration (4% v/v) and carbon levels (0.15% sucrose m/v) of extracted sample solutions produced consistent responses from plant material. High carbon concentrations (up to 8% sucrose m/v) caused matrix interferences that decreased blank values by 4.5 mg L-1 and suppressed Cl signals by 20%. No interferences from countercations were observed. A simple extraction process for dried plant samples (0.1 g in 40 mL of 4% HNO3) was developed and tested with plant standard reference materials. Recovery of Cl from the majority of materials was close to 100% of established values while relative standard deviations were below 4% except when chloride concentration was close to the method detection limit. The extraction method was robust to modifications for samples with low mass or high Cl concentrations.

    Original languageEnglish
    Pages (from-to)1946-1952
    Number of pages7
    JournalJournal of Analytical Atomic Spectrometry
    Issue number12
    Publication statusPublished - Dec 2010


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