Chromatin superstructure: The next level of structure above the nucleosome has an alternating character. A two-nucleosome based series is generated by probes armed with DNAase-I acting on isolated nuclei

L. A. Burgoyne; J. D. Skinner

doi:10.1016/0006-291X(81)91247-X

Chromatin superstructure: The next level of structure above the nucleosome has an alternating character. A two-nucleosome based series is generated by probes armed with DNAase-I acting on isolated nuclei

L. A. Burgoyne
, J. D. Skinner

College of Science and Engineering

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Intact nuclei were probed with DNAase-I-based probes and the resultant single strand pieces indicate that many or all of the nucleosomes exist in structures that cause every second nucleosome in a series to be resistant to attack. The effect is observed with chicken erythrocyte nuclei, rat liver nuclei and is weakly elicited by free DNAase-I. A method is described for the suppression of the intrinsic (CaMg) endonuclease of rat liver nuclei.

Original language	English
Pages (from-to)	893-899
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	99
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(81)91247-X
Publication status	Published - 15 Apr 1981

Access to Document

10.1016/0006-291X(81)91247-XLicence: In Copyright

Cite this

@article{f830f75d61ca4c0297bfccee25348e0f,

title = "Chromatin superstructure: The next level of structure above the nucleosome has an alternating character. A two-nucleosome based series is generated by probes armed with DNAase-I acting on isolated nuclei",

abstract = "Intact nuclei were probed with DNAase-I-based probes and the resultant single strand pieces indicate that many or all of the nucleosomes exist in structures that cause every second nucleosome in a series to be resistant to attack. The effect is observed with chicken erythrocyte nuclei, rat liver nuclei and is weakly elicited by free DNAase-I. A method is described for the suppression of the intrinsic (CaMg) endonuclease of rat liver nuclei.",

author = "Burgoyne, \{L. A.\} and Skinner, \{J. D.\}",

year = "1981",

month = apr,

day = "15",

doi = "10.1016/0006-291X(81)91247-X",

language = "English",

volume = "99",

pages = "893--899",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "3",

}

Chromatin superstructure: The next level of structure above the nucleosome has an alternating character. A two-nucleosome based series is generated by probes armed with DNAase-I acting on isolated nuclei. / Burgoyne, L. A.; Skinner, J. D.
In: Biochemical and Biophysical Research Communications, Vol. 99, No. 3, 15.04.1981, p. 893-899.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Chromatin superstructure

T2 - The next level of structure above the nucleosome has an alternating character. A two-nucleosome based series is generated by probes armed with DNAase-I acting on isolated nuclei

AU - Burgoyne, L. A.

AU - Skinner, J. D.

PY - 1981/4/15

Y1 - 1981/4/15

N2 - Intact nuclei were probed with DNAase-I-based probes and the resultant single strand pieces indicate that many or all of the nucleosomes exist in structures that cause every second nucleosome in a series to be resistant to attack. The effect is observed with chicken erythrocyte nuclei, rat liver nuclei and is weakly elicited by free DNAase-I. A method is described for the suppression of the intrinsic (CaMg) endonuclease of rat liver nuclei.

AB - Intact nuclei were probed with DNAase-I-based probes and the resultant single strand pieces indicate that many or all of the nucleosomes exist in structures that cause every second nucleosome in a series to be resistant to attack. The effect is observed with chicken erythrocyte nuclei, rat liver nuclei and is weakly elicited by free DNAase-I. A method is described for the suppression of the intrinsic (CaMg) endonuclease of rat liver nuclei.

UR - https://www.scopus.com/pages/publications/0019880872

U2 - 10.1016/0006-291X(81)91247-X

DO - 10.1016/0006-291X(81)91247-X

M3 - Article

C2 - 6264918

AN - SCOPUS:0019880872

SN - 0006-291X

VL - 99

SP - 893

EP - 899

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Chromatin superstructure: The next level of structure above the nucleosome has an alternating character. A two-nucleosome based series is generated by probes armed with DNAase-I acting on isolated nuclei

Abstract

Access to Document

Other files and links

Fingerprint

Cite this