Chromatin superstructure: The next level of structure above the nucleosome has an alternating character. A two-nucleosome based series is generated by probes armed with DNAase-I acting on isolated nuclei

L. A. Burgoyne; J. D. Skinner

doi:10.1016/0006-291X(81)91247-X

Chromatin superstructure: The next level of structure above the nucleosome has an alternating character. A two-nucleosome based series is generated by probes armed with DNAase-I acting on isolated nuclei

L. A. Burgoyne, J. D. Skinner

College of Science and Engineering

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38 Citations (Scopus)

Abstract

Intact nuclei were probed with DNAase-I-based probes and the resultant single strand pieces indicate that many or all of the nucleosomes exist in structures that cause every second nucleosome in a series to be resistant to attack. The effect is observed with chicken erythrocyte nuclei, rat liver nuclei and is weakly elicited by free DNAase-I. A method is described for the suppression of the intrinsic (CaMg) endonuclease of rat liver nuclei.

Original language	English
Pages (from-to)	893-899
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	99
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(81)91247-X
Publication status	Published - 15 Apr 1981

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Chromatin superstructure: The next level of structure above the nucleosome has an alternating character. A two-nucleosome based series is generated by probes armed with DNAase-I acting on isolated nuclei. / Burgoyne, L. A.; Skinner, J. D.
In: Biochemical and Biophysical Research Communications, Vol. 99, No. 3, 15.04.1981, p. 893-899.

Research output: Contribution to journal › Article › peer-review

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AB - Intact nuclei were probed with DNAase-I-based probes and the resultant single strand pieces indicate that many or all of the nucleosomes exist in structures that cause every second nucleosome in a series to be resistant to attack. The effect is observed with chicken erythrocyte nuclei, rat liver nuclei and is weakly elicited by free DNAase-I. A method is described for the suppression of the intrinsic (CaMg) endonuclease of rat liver nuclei.

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Chromatin superstructure: The next level of structure above the nucleosome has an alternating character. A two-nucleosome based series is generated by probes armed with DNAase-I acting on isolated nuclei

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